Universität zu Köln

Lalioti, Maria-Eleni, Arbi, Marina, Loukas, Ioannis, Kaplani, Konstantina ORCID: 0000-0003-4710-5494, Kalogeropoulou, Argyro, Lokka, Georgia, Kyrousi, Christina ORCID: 0000-0002-6234-8382, Mizi, Athanasia ORCID: 0000-0002-4313-491X, Georgomanolis, Theodore, Josipovic, Natasa, Gkikas, Dimitrios, Benes, Vladimir, Politis, Panagiotis K., Papantonis, Argyris, Lygerou, Zoi and Taraviras, Stavros ORCID: 0000-0002-7455-647X (2019). GemC1 governs multiciliogenesis through direct interaction with and transcriptional regulation of p73. J. Cell Sci., 132 (11). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9137

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Abstract

A distinct combination of transcription factors elicits the acquisition of a specific fate and the initiation of a differentiation program. Multiciliated cells (MCCs) are a specialized type of epithelial cells that possess dozens of motile cilia on their apical surface. Defects in cilia function have been associated with ciliopathies that affect many organs, including brain and airway epithelium. Here we show that the geminin coiled-coil domain-containing protein 1 GemC1 (also known as Lynkeas) regulates the transcriptional activation of p73, a transcription factor central to multiciliogenesis. Moreover, we show that GemC1 acts in a trimeric complex with transcription factor E2F5 and tumor protein p73 (officially known as TP73), and that this complex is important for the activation of the p73 promoter. We also provide in vivo evidence that GemC1 is necessary for p73 expression in different multiciliated epithelia. We further show that GemC1 regulates multiciliogenesis through the control of chromatin organization, and the epigenetic marks/tags of p73 and Foxj1. Our results highlight novel signaling cues involved in the commitment program of MCCs across species and tissues. This article has an associated First Person interview with the first author of the paper.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lalioti, Maria-Eleni UNSPECIFIED UNSPECIFIED UNSPECIFIED Arbi, Marina UNSPECIFIED UNSPECIFIED UNSPECIFIED Loukas, Ioannis UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaplani, Konstantina UNSPECIFIED orcid.org/0000-0003-4710-5494 UNSPECIFIED Kalogeropoulou, Argyro UNSPECIFIED UNSPECIFIED UNSPECIFIED Lokka, Georgia UNSPECIFIED UNSPECIFIED UNSPECIFIED Kyrousi, Christina UNSPECIFIED orcid.org/0000-0002-6234-8382 UNSPECIFIED Mizi, Athanasia UNSPECIFIED orcid.org/0000-0002-4313-491X UNSPECIFIED Georgomanolis, Theodore UNSPECIFIED UNSPECIFIED UNSPECIFIED Josipovic, Natasa UNSPECIFIED UNSPECIFIED UNSPECIFIED Gkikas, Dimitrios UNSPECIFIED UNSPECIFIED UNSPECIFIED Benes, Vladimir UNSPECIFIED UNSPECIFIED UNSPECIFIED Politis, Panagiotis K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Papantonis, Argyris UNSPECIFIED UNSPECIFIED UNSPECIFIED Lygerou, Zoi UNSPECIFIED UNSPECIFIED UNSPECIFIED Taraviras, Stavros UNSPECIFIED orcid.org/0000-0002-7455-647X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-146166
DOI:	10.1242/jcs.228684
Journal or Publication Title:	J. Cell Sci.
Volume:	132
Number:	11
Date:	2019
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GEMININ; CELLS; DIFFERENTIATION; EXPRESSION; PROTEIN; IDAS Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14616