Universität zu Köln

Jazmati, N., Hain, O., Hellmich, M., Plum, G. and Kaasch, A. (2019). PCR based detection of tcdC Delta 117 in Clostridium difficile infection identifies patients at risk for recurrence - A hospital-based prospective observational study. Anaerobe, 57. S. 39 - 45. OXFORD: ELSEVIER SCI LTD. ISSN 1095-8274

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Abstract

Objectives: Increasing incidence and severity of Clostridium difficile infection (CDI) in the last decades has been attributed to the emergence of hypervirulent C. difficile strain PCR-ribotype 027 (RT027). Commercial multiplex real-time PCR tests allow the presumptive identification of RT027 by detecting a single-base deletion at nt117 in the tcdC gene (tcdC Delta 117). The clinical usefulness of the detection of tcdC Delta 117 is unclear. Therefore, we evaluated test performance and clinical association of the detection of tcdC Delta 117 in patients with CDI in a prospective observational study conducted in a tertiary care hospital in Germany. Methods: From June to October 2015, stool from all patients with suspected CDI was tested for C. difficile according to ESCMID guidelines. C difficile was cultured from positive samples and ribotyping was performed. Clinical data were collected prospectively from all C. difficile positive patients. Results: From 1121 tested stool samples 107 patients with CDI were included in the study. tcdC Delta 117 was detected in 18 (16.8%) of these patients. Multivariable logistic regression analysis revealed an independent association of detection of tcdC Delta 117 with a further episode of CDI (OR 14.6; 95% CI 3.6-58.3: p < 0.001) and death within 30 days of the positive test (OR 5.1; 95% CI 1.0-25.7; p = 0.046). As follow up data are limited, it remains unclear, whether the further episode of CDI was due to tcdC Delta 117 (recurrence) or another type. Conclusion: In our setting, PCR-based detection of tcdC Delta 117 identified patients at risk for recurrent CDI and increased mortality and thus may guide therapeutic choices in CDI patients at the time of diagnosis. (C) 2019 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jazmati, N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hain, O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hellmich, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Plum, G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaasch, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-146190
DOI:	10.1016/j.anaerobe.2019.03.010
Journal or Publication Title:	Anaerobe
Volume:	57
Page Range:	S. 39 - 45
Date:	2019
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	1095-8274
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RIBOTYPE 027; NAP1 STRAIN; EPIDEMIOLOGY; SEVERITY; SOCIETY; DISEASE; UPDATE Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14619