Sachinidis, Agapios, Albrecht, Wiebke, Nell, Patrick ORCID: 0000-0002-1421-3248, Cherianidou, Anna, Hewitt, Nicola J., Edlund, Karolina and Hengstler, Jan G. (2019). Road Map for Development of Stem Cell-Based Alternative Test Methods. Trends Mol. Med, 25 (6). S. 470 - 482. OXFORD: ELSEVIER SCI LTD. ISSN 1471-499X

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Abstract

Much progress has been made in establishing strategies for differentiation of induced human pluripotent stem cells (hiPSCs). However, differentiated hiPSCs are not yet routinely use for prediction of toxicity. Here, limiting factors are summarised and possibilities for improvement are discussed, with a focus on hepatocytes, cardiomyocytes, tubular epithelial cells, and developmental tox-icity. Moreover, we make recommendation for further fine-tuning of differentiation protocols for hiPSCs to hepatocyte-like cells by comparing individual steps of currently available protocols to the mechanisms occurring during embryonic development. A road map is proposed to facilitate test system development, including a description of the most useful performance metrics.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Sachinidis, AgapiosUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Albrecht, WiebkeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nell, PatrickUNSPECIFIEDorcid.org/0000-0002-1421-3248UNSPECIFIED
Cherianidou, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hewitt, Nicola J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Edlund, KarolinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hengstler, Jan G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-146278
DOI: 10.1016/j.molmed.2019.04.003
Journal or Publication Title: Trends Mol. Med
Volume: 25
Number: 6
Page Range: S. 470 - 482
Date: 2019
Publisher: ELSEVIER SCI LTD
Place of Publication: OXFORD
ISSN: 1471-499X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
IN-VITRO SYSTEMS; PRIMARY HEPATOCYTES; NEPHROTOXIC COMPOUNDS; DRUG-METABOLISM; DIFFERENTIATION; HEPATOTOXICITY; PHARMACOGENETICS; WITHDRAWN; PROTEINS; DESIGNMultiple languages
Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & ExperimentalMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/14627

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