Universität zu Köln

Cerezales, Monica ORCID: 0000-0001-6705-006X, Xanthopoulou, Kyriaki ORCID: 0000-0001-8591-8184, Wille, Julia, Bustamante, Zulema, Seifert, Harald, Gallego, Lucia ORCID: 0000-0003-2885-7789 and Higgins, Paul G. ORCID: 0000-0001-8677-9454 (2019). Acinetobacter baumannii analysis by core genome multi-locus sequence typing in two hospitals in Bolivia: endemicity of international clone 7 isolates (CC25). Int. J. Antimicrob. Agents, 53 (6). S. 844 - 850. AMSTERDAM: ELSEVIER. ISSN 1872-7913

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Abstract

In total, 95 Acinetobacter baumannii isolates recovered from patients from two hospitals in Cochabamba, Bolivia were studied. The presence of class D and B beta-lactamases was investigated using polymerase chain reaction, and antimicrobial susceptibility testing was performed by agar dilution and broth microdilution. The resistance rate to carbapenems was 53.7%. All carbapenem-resistant A. baumannii (CRAb, n = 51) and four carbapenem-susceptible isolates were further analysed by whole-genome sequencing. The resulting genome assemblies were used to identify the acquired resistome, and core genome multi-locus sequence typing (cgMLST) was used to determine their molecular epidemiology. All but one of the CRAb isolates (n = 50) belonged to international clone (IC) 7 and they clustered into five sequence types; on cgMLST, they were found to be separated by >= 40 alleles. All CRAb isolates carried bla(OXA-23) on transposon Tn2008. Metallo-beta-lactamases were not detected. These data show that dissemination of several IC7 A. baumannii clones harbouring the carbapenem resistance determinant bla(OXA-23) is occurring in these two hospitals in Cochambamba. (C) 2019 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Cerezales, Monica UNSPECIFIED orcid.org/0000-0001-6705-006X UNSPECIFIED Xanthopoulou, Kyriaki UNSPECIFIED orcid.org/0000-0001-8591-8184 UNSPECIFIED Wille, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Bustamante, Zulema UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Gallego, Lucia UNSPECIFIED orcid.org/0000-0003-2885-7789 UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-147081
DOI:	10.1016/j.ijantimicag.2019.03.019
Journal or Publication Title:	Int. J. Antimicrob. Agents
Volume:	53
Number:	6
Page Range:	S. 844 - 850
Date:	2019
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1872-7913
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MOLECULAR EPIDEMIOLOGY; IDENTIFICATION; CALCOACETICUS; RESISTANCE; PLASMID Multiple languages Infectious Diseases; Microbiology; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14708