Universität zu Köln

Verhasselt, Hedda Luise, Radke, Julia, Schmidt, Dirk, Killengray, David, Scharmann, Ulrike, Rickerts, Volker, Hansen, Wiebke, Seidel, Danila, Falces-Romero, Iker, Buer, Jan ORCID: 0000-0002-7602-1698, Rath, Peter-Michael and Steinmann, Joerg (2019). Comparison of genotyping methods for Cunninghamella bertholletiae. Mycoses, 62 (6). S. 519 - 526. HOBOKEN: WILEY. ISSN 1439-0507

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Abstract

Background Invasive fungal infections caused by filamentous fungi of the order Mucorales are serious complications in immunocompromised patients and often associated with fatal outcome. As a member of this order, Cunninghamella bertholletiae is a saprophytic fungus with naturally exhibited high minimum inhibitory concentrations against common antifungal drugs and with the potential for outbreaks in clinical settings. Objectives and methods In a proof-of-principle study, we evaluated the performance of microsatellite markers for the discrimination of thirteen C. bertholletiae isolates from various sources in comparison with a repetitive sequence-based PCR (rep-PCR) and random amplification of polymorphic DNA (RAPD). Based on the higher discriminatory power of the microsatellite PCR with five separate primer pairs (Simpson's index of 1 vs 0 [RAPD] and 0 [rep-PCR]), the novel method was applied to eight additional isolates, including four well-characterised isolates from a cluster of infections in a next step. Results In total, microsatellite PCR identified 21 separate genotypes. A probable epidemiological association of the cluster isolates could be demonstrated by microsatellite genotyping. Conclusion In conclusion, our findings demonstrate the value of microsatellite PCR in genotyping Cunninghamella bertholletiae and its potential for future applications with other species of the order Mucorales.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Verhasselt, Hedda Luise UNSPECIFIED UNSPECIFIED UNSPECIFIED Radke, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED Killengray, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Scharmann, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Rickerts, Volker UNSPECIFIED UNSPECIFIED UNSPECIFIED Hansen, Wiebke UNSPECIFIED UNSPECIFIED UNSPECIFIED Seidel, Danila UNSPECIFIED UNSPECIFIED UNSPECIFIED Falces-Romero, Iker UNSPECIFIED UNSPECIFIED UNSPECIFIED Buer, Jan UNSPECIFIED orcid.org/0000-0002-7602-1698 UNSPECIFIED Rath, Peter-Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Steinmann, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-147393
DOI:	10.1111/myc.12908
Journal or Publication Title:	Mycoses
Volume:	62
Number:	6
Page Range:	S. 519 - 526
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1439-0507
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INVASIVE ASPERGILLOSIS; MUCORMYCOSIS; DIVERSITY Multiple languages Dermatology; Mycology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14739