Universität zu Köln

Happe, Arndt, Sielker, Sonja, Hanisch, Marcel and Jung, Susanne (2019). The Biologic Effect of Particulate Titanium Contaminants of Dental Implants on Human Osteoblasts and Gingival Fibroblasts. Int. J. Oral Maxillofac. Implants, 34 (3). S. 673 - 681. HANOVER PARK: QUINTESSENCE PUBLISHING CO INC. ISSN 1942-4434

Full text not available from this repository.

Abstract

Purpose: To evaluate the effects of different titanium particle concentrations on the viability of human calvarial osteoblasts and human gingival fibroblasts. Materials and Methods: Primary human calvarial osteoblasts (HCO, 3H Biomedical) and human gingival fibroblasts (HGF-1, ATCC) were cultivated and allowed to adhere for 24 hours. Titanium powder concentrations (0.01 to 1.0 mg/mL) were added, and samples were analyzed at three time points (24 hours, 7 days, 21 days). Cell viability was analyzed using living cell count, proliferation (MTT) assay, and live/dead staining. Cytotoxic effects were evaluated using lactated dehydrogenase assay. Qualitative analysis of cell viability was performed. In addition, scanning electron microscopy (SEM) analysis was performed. Release of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) was estimated with human IL-6/human TNF-alpha ELISA. Results: Titanium concentrations of 0.1 mg/mL and 1.0 mg/mL showed medium-and long-term effects on cell growth and proliferation rates. Cytotoxic effects by release of lactate dehydrogenase were observable during the first 24 hours. Human gingival fibroblast cells showed a release factor between 2.6 and 3.4. Titanium powder seemed to be more cytotoxic to human gingival fibroblast cells than to human calvarial osteoblast cells. For human calvarial osteoblasts, only the highest concentration showed cytotoxic effects with a release factor of 2.7. Human calvarial osteoblasts secreted IL-6 only during the first 24 hours and only in the highest titanium concentration, whereas human gingival fibroblasts secreted IL-6 during the entire period. The lowest titanium concentration showed stronger secretion of IL-6 compared with the control. Incorporation of smaller and single titanium particles by cells was identified under SEM analysis. Conclusion: Cell viability is negatively correlated with titanium concentration. Further, titanium debris might lead to an inflammatory biologic response of dental peri-implant tissue. Also, cells interact with the debris, eg, with incorporation of particles.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Happe, Arndt UNSPECIFIED UNSPECIFIED UNSPECIFIED Sielker, Sonja UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanisch, Marcel UNSPECIFIED UNSPECIFIED UNSPECIFIED Jung, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-148816
DOI:	10.11607/jomi.6929
Journal or Publication Title:	Int. J. Oral Maxillofac. Implants
Volume:	34
Number:	3
Page Range:	S. 673 - 681
Date:	2019
Publisher:	QUINTESSENCE PUBLISHING CO INC
Place of Publication:	HANOVER PARK
ISSN:	1942-4434
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ABUTMENT INTERFACE; HISTOMETRIC EVALUATION; SUBMERGED IMPLANTS; PERI-IMPLANTITIS; WEAR; PARTICLES; PHAGOCYTOSIS; CONNECTIONS; MACROPHAGES; DEBRIS Multiple languages Dentistry, Oral Surgery & Medicine Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14881