Universität zu Köln

Lundt, Andreas, Soos, Julien, Henseler, Christina, Arshaad, Muhammad Imran, Mueller, Ralf, Ehninger, Dan, Hescheler, Juergen, Sachinidis, Agapios, Broich, Karl, Wormuth, Carole, Papazoglou, Anna and Weiergraeber, Marco (2019). Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice. J. Vis. Exp. (147). CAMBRIDGE: JOURNAL OF VISUALIZED EXPERIMENTS. ISSN 1940-087X

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Abstract

Brainstem evoked response audiometry (BERA) is of central relevance in the clinical neurophysiology. As other evoked potential (EP) techniques, such as visually evoked potentials (VEPs) or somatosensory evoked potentials (SEPs), the auditory evoked potentials (AEPs) are triggered by the repetitive presentation of identical stimuli, the electroencephalographic (EEG) response of which is subsequently averaged resulting in distinct positive (p) and negative (n) deflections. In humans, both the amplitude and the latency of individual peaks can be used to characterize alterations in synchronization and conduction velocity in the underlying neuronal circuitries. Importantly, AEPs are also applied in basic and preclinical science to identify and characterize the auditory function in pharmacological and genetic animal models. Even more, animal models in combination with pharmacological testing are utilized to investigate for potential benefits in the treatment of sensorineural hearing loss (e.g., age-or noise-induced hearing deficits). Here we provide a detailed and integrative description of how to record auditory brainstem-evoked responses (ABRs) in mice using click and tone-burst application. A specific focus of this protocol is on pre-experimental animal housing, anesthesia, ABR recording, ABR filtering processes, automated wavelet-based amplitude growth function analysis, and latency detection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lundt, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Soos, Julien UNSPECIFIED UNSPECIFIED UNSPECIFIED Henseler, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Arshaad, Muhammad Imran UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Ralf UNSPECIFIED UNSPECIFIED UNSPECIFIED Ehninger, Dan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Broich, Karl UNSPECIFIED UNSPECIFIED UNSPECIFIED Wormuth, Carole UNSPECIFIED UNSPECIFIED UNSPECIFIED Papazoglou, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Weiergraeber, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-149144
DOI:	10.3791/59200
Journal or Publication Title:	J. Vis. Exp.
Number:	147
Date:	2019
Publisher:	JOURNAL OF VISUALIZED EXPERIMENTS
Place of Publication:	CAMBRIDGE
ISSN:	1940-087X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MULTIELECTRODE ARRAY; INBRED STRAINS; MOUSE MODELS; HEARING-LOSS; POTENTIALS; SCHIZOPHRENIA; STIMULATION; ANESTHESIA; SPECTRUM; DEAFNESS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14914