Universität zu Köln

Annibal, Andrea ORCID: 0000-0003-4311-5417, Tam, Hannah, Latza, Christian and Antebi, Adam (2019). Comparison of ESI-MS/MS and APCI-MS methods for the quantification of folic acid analogs in C. elegans. J. Mass Spectrom., 54 (4). S. 316 - 328. HOBOKEN: WILEY. ISSN 1096-9888

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Abstract

Folic acid (FA) plays a vital role in central metabolism, including the one carbon cycle, nucleotide, and amino acid biosynthesis. The development of sensitive, accurate analytical methods to measure FA intermediates in tissues is critical to understand their biological roles in diverse physiological and pathological contexts. Here, we developed a highly sensitive method for the simultaneous quantification of FA intermediates in the nematode Caenorhabditis elegans as a model to dissect metabolic networks. The method was further validated by analyzing the worm folate pool upon RNAi knockdown of the dihydrofolate reductase gene dhfr-1. Comparative mass spectrometry behavior of the FA analogs using two different ion sources, electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), revealed ESI-MS/MS to be more sensitive, but APCI-MS provided more detailed structure inferences, which can elucidate chemical investigation and synthesis of FA analogs. Finally, we report on the use of in vitro oxidation coupled with high-resolution mass spectrometry as a tool to discover new endogenous FA derivatives in the nematode.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Annibal, Andrea UNSPECIFIED orcid.org/0000-0003-4311-5417 UNSPECIFIED Tam, Hannah UNSPECIFIED UNSPECIFIED UNSPECIFIED Latza, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Antebi, Adam UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-151930
DOI:	10.1002/jms.4337
Journal or Publication Title:	J. Mass Spectrom.
Volume:	54
Number:	4
Page Range:	S. 316 - 328
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1096-9888
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ONE-CARBON METABOLISM; TETRAHYDROFOLIC ACID; MASS-SPECTROMETRY; FOLATE; ELECTROSPRAY; METHOTREXATE; DEFICIENCY; MECHANISMS; HYPERHOMOCYSTEINEMIA; SUPPLEMENTATION Multiple languages Biochemical Research Methods; Chemistry, Analytical; Spectroscopy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15193