Universität zu Köln

Springer, J., Smith, I. McCormick, Hartmann, S., Winkelmann, R., Wilmes, D., Cornely, O., Kessel, J., Loeffler, J. and Rickerts, V. (2019). Identification of Aspergillus and Mucorales in formalin-fixed, paraffin-embedded tissue samples: Comparison of specific and broad-range fungal qPCR assays. Med. Mycol., 57 (3). S. 308 - 314. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2709

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Abstract

Establishing the etiology of invasive fungal infections is important to guide therapeutic options and for epidemiologic purposes. Formalin-fixed, paraffin-embedded (FFPE) tissue specimens from patients with proven invasive fungal infections are valuable to determine the etiology of systemic fungal infections. We compared different polymerase chain reaction (PCR) amplification strategies from FFPE tissue blocks to identify agents of invasive fungal infections. We found that specific PCR assays show superior sensitivity in the identification of DNA of Mucorales and Aspergillus and mixed infections caused by both as compared to broad-range PCR assays. Shorter amplicon lengths and less detection of contaminating fungal DNA are potential factors involved. However, detection of fungal DNA by highly sensitive specific PCR assays in the absence of demonstration of fungal elements in tissue suggests that PCR results should be interpreted in the context of the histopathology and clinical findings.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Springer, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Smith, I. McCormick UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Winkelmann, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wilmes, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cornely, O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kessel, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Loeffler, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rickerts, V. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-152283
DOI:	10.1093/mmy/myy041
Journal or Publication Title:	Med. Mycol.
Volume:	57
Number:	3
Page Range:	S. 308 - 314
Date:	2019
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2709
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PCR ASSAYS; HIGH-RISK; DNA Multiple languages Infectious Diseases; Mycology; Veterinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15228