Rossen, John W. A., Dombrecht, Jill, Vanfleteren, Diederik, De Bruyne, Katrien, van Belkum, Alex, Rosema, Sigrid, Lokate, Mariette, Bathoorn, Erik, Reuter, Sandra, Grundmann, Hajo, Ertel, Julia, Higgins, Paul G. ORCID: 0000-0001-8677-9454 and Seifert, Harald (2019). Epidemiological Typing of Serratia marcescens Isolates by Whole-Genome Multilocus Sequence Typing. J. Clin. Microbiol., 57 (4). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-660X

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Abstract

Serratia marcescens is an opportunistic bacterial pathogen. It is notorious for its increasing antimicrobial resistance and its potential to cause outbreaks of colonization and infections, predominantly in neonatal intensive care units (NICUs). There, its spread requires rapid infection control response. To understand its spread, detailed molecular typing is key. We present a whole-genome multilocus sequence typing (wgMLST) method for S. marcescens. Using a set of 299 publicly available whole-genome sequences (WGS), we developed an initial wgMLST system consisting of 9,377 gene loci. This included 1,455 loci occurring in all reference genomes and 7,922 accessory loci. This closed system was validated using three geographically diverse collections of S. marcescens consisting of 111 clinical isolates implicated in nosocomial dissemination events in three hospitals. The validation procedure showed a full match between epidemiological data and the wgMLST analyses. We set the cut- off value for epidemiological (non)relatedness at 20 different alleles, though for the majority of outbreak-clustered isolates, this difference was limited to 4 alleles. This shows that the wgMLST system for S. marcescens provides prospects for successful future monitoring for the epidemiological containment of this opportunistic pathogen.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Rossen, John W. A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dombrecht, JillUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vanfleteren, DiederikUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
De Bruyne, KatrienUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van Belkum, AlexUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rosema, SigridUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lokate, MarietteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bathoorn, ErikUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Reuter, SandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grundmann, HajoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ertel, JuliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Higgins, Paul G.UNSPECIFIEDorcid.org/0000-0001-8677-9454UNSPECIFIED
Seifert, HaraldUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-152492
DOI: 10.1128/JCM.01652-18
Journal or Publication Title: J. Clin. Microbiol.
Volume: 57
Number: 4
Date: 2019
Publisher: AMER SOC MICROBIOLOGY
Place of Publication: WASHINGTON
ISSN: 1098-660X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
INTENSIVE-CARE-UNIT; HOSPITAL HYGIENE; OUTBREAK; DISEASE; INFECTIONSMultiple languages
MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/15249

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