Capri, Miriam, Morsiani, Cristina, Santoro, Aurelia, Moriggi, Manuela, Conte, Maria, Martucci, Morena, Bellavista, Elena, Fabbri, Cristina, Giampieri, Enrico, Albracht, Kirsten ORCID: 0000-0002-4271-2511, Fluck, Martin, Ruoss, Severin ORCID: 0000-0001-8896-635X, Brocca, Lorenza, Canepari, Monica, Longa, Emanuela, Di Giulio, Irene, Bottinelli, Roberto, Cerretelli, Paolo, Salvioli, Stefano, Gelfi, Cecilia, Franceschi, Claudio, Narici, Marco and Rittweger, Joern (2019). Recovery from 6-month spaceflight at the International Space Station: muscle-related stress into a proinflammatory setting. Faseb J., 33 (4). S. 5168 - 5181. BETHESDA: FEDERATION AMER SOC EXP BIOL. ISSN 1530-6860

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Abstract

The Sarcolab pilot study of 2 crewmembers, investigated before and after a 6-mo International Space Station mission, has demonstrated the substantial muscle wasting and weakness, along with disruption of muscle's oxidative metabolism. The present work aimed at evaluating the pro/anti-inflammatory status in the same 2 crewmembers (A, B). Blood circulating (c-)microRNAs (miRs), c-proteasome, c-mitochondrial DNA, and cytokines were assessed by real-time quantitative PCR or ELISA tests. Time series analysis was performed (i.e., before flight and after landing) at 1 and 15 d of recovery (R+1 and R+15, respectively). C-biomarkers were compared with an age-matched control population and with 2-dimensional proteomic analysis of the 2 crewmembers' muscle biopsies. Striking differences were observed between the 2 crewmembers at R+1, in terms of inflamma-miRs (c-miRs-21-5p, -126-3p, and -146a-5p), muscle specific (myo)-miR-206, c-proteasome, and IL-6/leptin, thus making the 2 astronauts dissimilar to each other. Final recovery levels of c-proteasome, c-inflamma-miRs, and c-myo-miR-206 were not reverted to the baseline values in crewmember A. In both crewmembers, myo-miR-206 changed significantly after recovery. Muscle biopsy of astronaut A showed an impressive 80% increase of alpha-1-antitrypsin, a target of miR-126-3p. These results point to a strong stress response induced by spaceflight involving muscle tissue and the proinflammatory setting, where inflamma-miRs and myo-miR-206 mediate the systemic recovery phase after landing.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Capri, MiriamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Morsiani, CristinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Santoro, AureliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Moriggi, ManuelaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Conte, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Martucci, MorenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bellavista, ElenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fabbri, CristinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Giampieri, EnricoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Albracht, KirstenUNSPECIFIEDorcid.org/0000-0002-4271-2511UNSPECIFIED
Fluck, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ruoss, SeverinUNSPECIFIEDorcid.org/0000-0001-8896-635XUNSPECIFIED
Brocca, LorenzaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Canepari, MonicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Longa, EmanuelaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Di Giulio, IreneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bottinelli, RobertoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cerretelli, PaoloUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Salvioli, StefanoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gelfi, CeciliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Franceschi, ClaudioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Narici, MarcoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rittweger, JoernUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-152601
DOI: 10.1096/fj.201801625R
Journal or Publication Title: Faseb J.
Volume: 33
Number: 4
Page Range: S. 5168 - 5181
Date: 2019
Publisher: FEDERATION AMER SOC EXP BIOL
Place of Publication: BETHESDA
ISSN: 1530-6860
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CIRCULATING MICRORNAS; SKELETAL-MUSCLE; INFLAMMATION; SENESCENCE; EXPRESSION; LONGEVITY; RELEVANCE; EXERCISE; TISSUES; MIR-21Multiple languages
Biochemistry & Molecular Biology; Biology; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/15260

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