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Zhang, Bipei and Huelskamp, Martin (2019). Evolutionary Analysis of MBW Function by Phenotypic Rescue in Arabidopsis thaliana. Front. Plant Sci., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

The MBW complex consisting of the three proteins R2R3MYB, bHLH and WDR regulates five traits in Arabidopsis thaliana including trichome and root hair patterning, seed coat color, anthocyanidin production and seed coat mucilage release. The WDR gene TTG1 regulates each trait in specific combinations with different bHLH and R2R3MYB proteins. In this study we analyze to what extent the biochemical properties of the MBW proteins contribute to trait specificity by expressing them in appropriate A. thaliana mutants. We show that the rescue behavior of A. thaliana bHLH and R2R3MYB protein is sufficient to explain the function as derived previously from mutant analysis. When extending this rescue approach using MBW proteins from other species we find that proteins involved in anthocyanidin regulation typically show a rescue of the anthocyanidin phenotype but not of the other traits. Finally, we correlate the rescue abilities of MBW protein from different species with the A. thaliana proteins.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhang, Bipei UNSPECIFIED UNSPECIFIED UNSPECIFIED Huelskamp, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-153261
DOI:	10.3389/fpls.2019.00375
Journal or Publication Title:	Front. Plant Sci.
Volume:	10
Date:	2019
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-462X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRICHOME DEVELOPMENT; MYB DOMAIN; ANTHOCYANIN BIOSYNTHESIS; TRANSCRIPTION FACTORS; REGULATORY GENE; MAIZE C1; PROTEIN; BHLH; IDENTIFICATION; ENCODES Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15326