Universität zu Köln

de la Torre, Natanael Vinegra, Kaschani, Farnusch, Kaiser, Markus, van der Hoorn, Renier A. L., Soppe, Wim J. J. and Villamil, Johana C. Misas (2019). Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds. Biochem. J., 476. S. 843 - 858. LONDON: PORTLAND PRESS LTD. ISSN 1470-8728

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Abstract

Seed quality is affected by different constituents of the seed. In general, seed lots are considered to be of high quality when they exhibit fast and homogeneous germination. When seeds are stored, they undergo different degrees of damage that have detrimental effects on their quality. Therefore, accurate prediction of the seed quality and viability levels of a seed lot is of high importance in the seed-producing industry. Here, we describe the use of activity-based protein profiling of proteases to evaluate the quality of artificially and naturally aged seeds of Arabidopsis thaliana. Using this approach, we have identified two protease activities with opposite behaviours in aged seeds of Arabidopsis that correlate with the quality status of the seeds. We show that vacuolar processing enzymes (VPEs) become more active during the ageing process, in both artificial and natural ageing treatments. Secondly, we demonstrate that serine hydrolases are active at the beginning of our artificial ageing treatment, but their labelling decreases along with seed viability. We present a list of candidate hydrolases active during seed germination and propose that these protease activities can be used in combination with VPEs to develop novel markers of seed quality.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code de la Torre, Natanael Vinegra UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaschani, Farnusch UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaiser, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Hoorn, Renier A. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Soppe, Wim J. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Villamil, Johana C. Misas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-153663
DOI:	10.1042/BCJ20180911
Journal or Publication Title:	Biochem. J.
Volume:	476
Page Range:	S. 843 - 858
Date:	2019
Publisher:	PORTLAND PRESS LTD
Place of Publication:	LONDON
ISSN:	1470-8728
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VACUOLAR PROCESSING ENZYME; PROGRAMMED CELL-DEATH; MAILLARD REACTIONS; STORAGE PROTEINS; PROTEASE ACTIVITIES; LIPID-PEROXIDATION; SUGAR HYDROLYSIS; GERMINATION; VIGOR; VPE Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15366