Universität zu Köln

Reinhardt, Timm, van Schingen, Mona, Windisch, Heidrun Sigrid, Ziegler, Thomas and Fink, Patrick ORCID: 0000-0002-5927-8977 (2019). Monitoring a loss: Detection of the semi-aquatic crocodile lizard (Shinisaurus crocodilurus) in inaccessible habitats via environmental DNA. Aquat. Conserv.-Mar. Freshw. Ecosyst., 29 (3). S. 353 - 361. HOBOKEN: WILEY. ISSN 1099-0755

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Abstract

Assessing the conservation status of a species is strongly dependent upon data on species distribution and abundance. With the emergence of novel methods for species monitoring - such as the use of environmental DNA (eDNA) - monitoring success can be improved at reduced expenditure in the field, particularly in remote regions and terrains where access is difficult or dangerous. The highly endangered crocodile lizard (Shinisaurus crocodilurus Ahl, 1930) inhabits fragmented sites of the remaining evergreen forest with running water systems in a narrow distribution range in southern China and north-east Vietnam. Crocodile lizards spend most of the day within or above water bodies, which are commonly remote and inaccessible. To monitor recent spatial occurrences, and to confirm the persistence or extinction of previously reported populations (especially in heavily altered habitats), the suitability of using eDNA and quantitative polymerase chain reaction (qPCR) was tested as an alternative method for monitoring this semiaquatic lizard. To assess the accuracy and limitations of this method, eDNA results from the field were compared with eDNA data from mesocosms and census data on the actual abundance of this species in the field. Environmental DNA of the crocodile lizard was detected in all of the positive controls, and in four of six natural sites; thus, all data collected using traditional field surveys were confirmed with eDNA results. eDNA monitoring was found to be a reliable method for assessing the viability of populations; we suggest that it should be developed as a tool for efficient wildlife management, particularly under difficult field and funding conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Reinhardt, Timm UNSPECIFIED UNSPECIFIED UNSPECIFIED van Schingen, Mona UNSPECIFIED UNSPECIFIED UNSPECIFIED Windisch, Heidrun Sigrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Ziegler, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Patrick UNSPECIFIED orcid.org/0000-0002-5927-8977 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-155024
DOI:	10.1002/aqc.3038
Journal or Publication Title:	Aquat. Conserv.-Mar. Freshw. Ecosyst.
Volume:	29
Number:	3
Page Range:	S. 353 - 361
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1099-0755
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LUOKENG NATURE-RESERVE; POPULATION TRENDS; EDNA; CONSERVATION; VIETNAM; TOOL; SURVEILLANCE; BIODIVERSITY; PROJECTS; REPTILES Multiple languages Environmental Sciences; Marine & Freshwater Biology; Water Resources Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15502