Universität zu Köln

Bhandari, Deepak D., Lapin, Dmitry ORCID: 0000-0001-9591-3950, Kracher, Barbara, von Born, Patrick, Bautor, Jaqueline, Niefind, Karsten ORCID: 0000-0002-0183-6315 and Parker, Jane E. (2019). An EDS1 heterodimer signalling surface enforces timely reprogramming of immunity genes in Arabidopsis. Nat. Commun., 10. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Plant intracellular NLR receptors recognise pathogen interference to trigger immunity but how NLRs signal is not known. Enhanced disease susceptibility1 (EDS1) heterodimers are recruited by Toll-interleukin1-receptor domain NLRs (TNLs) to transcriptionally mobilise resistance pathways. By interrogating the Arabidopsis EDS1 alpha-helical EP-domain we identify positively charged residues lining a cavity that are essential for TNL immunity signalling, beyond heterodimer formation. Mutating a single, conserved surface arginine (R493) disables TNL immunity to an oomycete pathogen and to bacteria producing the virulence factor, coronatine. Plants expressing a weakly active EDS1(R493A) variant have delayed transcriptional reprogramming, with severe consequences for resistance and countering bacterial coronatine repression of early immunity genes. The same EP-domain surface is utilised by a non-TNL receptor RPS2 for bacterial immunity, indicating that the EDS1 EP-domain signals in resistance conferred by different NLR receptor types. These data provide a unique structural insight to early downstream signalling in NLR receptor immunity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bhandari, Deepak D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lapin, Dmitry UNSPECIFIED orcid.org/0000-0001-9591-3950 UNSPECIFIED Kracher, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED von Born, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED Bautor, Jaqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED Niefind, Karsten UNSPECIFIED orcid.org/0000-0002-0183-6315 UNSPECIFIED Parker, Jane E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-156563
DOI:	10.1038/s41467-019-08783-0
Journal or Publication Title:	Nat. Commun.
Volume:	10
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SALICYLIC-ACID; PATHOGEN EFFECTORS; DEFENSE RESPONSES; STRUCTURAL BASIS; RECEPTOR RX1; CELL-DEATH; RESISTANCE; PAD4; RECOGNITION; BACTERIAL Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15656