Universität zu Köln

Ly, Alice, Ragionieri, Lapo, Liessem, Sander ORCID: 0000-0002-7073-2659, Becker, Michael, Deininger, Soeren-Oliver, Neupert, Susanne and Predel, Reinhard (2019). Enhanced Coverage of Insect Neuropeptides in Tissue Sections by an Optimized Mass-Spectrometry-Imaging Protocol. Anal. Chem., 91 (3). S. 1980 - 1989. WASHINGTON: AMER CHEMICAL SOC. ISSN 1520-6882

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Abstract

Mass spectrometry imaging (MSI) of neuropeptides has become a well-established method with the ability to combine spatially resolved information from immunohistochemistry with peptidomics information from mass spectrometric analysis. Several studies have conducted MSI of insect neural tissues; however, these studies did not detect neuropeptide complements in manners comparable to those of conventional peptidomics. The aim of our study was to improve sample preparation so that MSI could provide comprehensive and reproducible neuropeptidomics information. Using the cockroach retrocerebral complex, the presented protocol produces enhanced coverage of neuropeptides at 15 pm spatial resolution, which was confirmed by parallel analysis of tissue extracts using electrospray-ionization MS. Altogether, more than 100 peptide signals from 15 neuropeptide-precursor genes could be traced with high spatial resolution. In addition, MSI spectra confirmed differential prohormone processing and distinct neuropeptide-based compartmentalization of the retrocerebral complex. We believe that our workflow facilitates incorporation of MSI in neuroscience-related topics, including the study of complex neuropeptide interactions within the CNS.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ly, Alice UNSPECIFIED UNSPECIFIED UNSPECIFIED Ragionieri, Lapo UNSPECIFIED UNSPECIFIED UNSPECIFIED Liessem, Sander UNSPECIFIED orcid.org/0000-0002-7073-2659 UNSPECIFIED Becker, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Deininger, Soeren-Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Neupert, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-156928
DOI:	10.1021/acs.analchem.8b04304
Journal or Publication Title:	Anal. Chem.
Volume:	91
Number:	3
Page Range:	S. 1980 - 1989
Date:	2019
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1520-6882
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COCKROACH DIPLOPTERA-PUNCTATA; NERVOUS-SYSTEM; RETROCEREBRAL COMPLEX; NEURONAL PATHWAYS; MALDI-MS; EXPRESSION; PEPTIDES; BRAIN; IDENTIFICATION; PEPTIDOMICS Multiple languages Chemistry, Analytical Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15692