Universität zu Köln

Yazdanpanah, Farzaneh ORCID: 0000-0001-7768-4621, Maurino, Veronica G., Mettler-Altmann, Tabea, Buijs, Gonda, Bailly, Marlene, Jashni, Mansoor Karimi, Willems, Leo, Sergeeva, Lidiya I., Rajjou, Loic ORCID: 0000-0001-9739-1041, Hilhorst, Henk W. M. and Bentsink, Leonie ORCID: 0000-0001-9510-6059 (2019). NADP-MALIC ENZYME 1 Affects Germination after Seed Storage in Arabidopsis thaliana. Plant Cell Physiol., 60 (2). S. 318 - 329. OXFORD: OXFORD UNIV PRESS. ISSN 1471-9053

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Abstract

Aging decreases the quality of seeds and results in agricultural and economic losses. The damage that occurs at the biochemical level can alter the seed physiological status. Although loss of viability has been investigated frequently, little information exists on the molecular and biochemical factors involved in seed deterioration and loss of viability. Oxidative stress has been implicated as a major contributor to seed deterioration, and several pathways are involved in protection against this. In this study, we show that seeds of Arabidopsis thaliana lacking a functional NADP-MALIC ENZYME 1 (NADP-ME1) have reduced seed viability relative to the wild type. Seeds of the NADP-ME1 loss-of-function mutant display higher levels of protein carbonylation than those of the wild type. NADP-ME1 catalyzes the oxidative decarboxylation of malate to pyruvate with the simultaneous production of CO2 and NADPH. Upon seed imbibition, malate and amino acids accumulate in embryos of aged seeds of the NADP-ME1 loss-of-function mutant compared with those of the wild type. NADP-ME1 expression is increased in imbibed aged as compared with non-aged seeds. NADP-ME1 activity at testa rupture promotes normal germination of aged seeds. In seedlings of aged seeds, NADP-ME1 is specifically active in the root meristematic zone. We propose that NADP-ME1 activity is required for protecting seeds against oxidation during seed dry storage.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Yazdanpanah, Farzaneh UNSPECIFIED orcid.org/0000-0001-7768-4621 UNSPECIFIED Maurino, Veronica G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mettler-Altmann, Tabea UNSPECIFIED UNSPECIFIED UNSPECIFIED Buijs, Gonda UNSPECIFIED UNSPECIFIED UNSPECIFIED Bailly, Marlene UNSPECIFIED UNSPECIFIED UNSPECIFIED Jashni, Mansoor Karimi UNSPECIFIED UNSPECIFIED UNSPECIFIED Willems, Leo UNSPECIFIED UNSPECIFIED UNSPECIFIED Sergeeva, Lidiya I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rajjou, Loic UNSPECIFIED orcid.org/0000-0001-9739-1041 UNSPECIFIED Hilhorst, Henk W. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bentsink, Leonie UNSPECIFIED orcid.org/0000-0001-9510-6059 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-157459
DOI:	10.1093/pcp/pcy213
Journal or Publication Title:	Plant Cell Physiol.
Volume:	60
Number:	2
Page Range:	S. 318 - 329
Date:	2019
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1471-9053
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN OXIDATION; LONGEVITY; DORMANCY; OXYGEN; EXPRESSION; MATURATION; GENES; VIGOR; IDENTIFICATION; ACCUMULATION Multiple languages Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15745