Universität zu Köln

Langenbach, Dorothee and Melkonian, Michael (2019). Optimising biomass and peridinin accumulation in the dinoflagellate Symbiodinium voratum using a twin-layer porous substrate bioreactor. J. Appl. Phycol., 31 (1). S. 21 - 29. DORDRECHT: SPRINGER. ISSN 1573-5176

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Abstract

Dinoflagellates are a natural source for unique secondary metabolites and pigments, which have considerable potential for applications in biomedical drug development and cosmetics. However, the technical cultivation of dinoflagellates in commonly used suspension-based closed photobioreactors (PBRs) has been shown to be difficult due to the sensitivity of the cultures to turbulence and shear forces. To overcome these and other constraints of suspension cultivation, immobilised cultivation of the dinoflagellate Symbiodinium voratum has been performed using a twin-layer porous substrate bioreactor (TL-PSBR). By optimising biomass growth using printing paper as substrate, a maximal biomass growth rate of 7.8g dry weight m(-2) growth area day(-1) was measured over a period of 40days at the relatively high-light intensity of 600mol photons m(-2)s(-1) and 2% (v/v) CO2. Linear growth of the S. voratum biofilm was observed over 40days, and a maximal biomass standing crop of 305gm(-2) was gained. Synthesis of the potentially high-value carotenoid pigment peridinin, however, was favoured at low-light conditions (100mol photons m(-2)s(-1)). By combining maximal biomass growth at high-light conditions with maximal peridinin accumulation at low-light conditions in a two-phase approach (14days cultivation at 600mol photons m(-2)s(-1) and 2% CO2 followed by 14days at 100mol photons m(-2)s(-1) and ambient air), a peridinin productivity of 51.4mg peridinin m(-2)day(-1) was measured (about 30% higher than the values determined at either low- or high-light conditions). Using the two-phase approach, peridinin accumulated to a standing crop of similar to 1g peridinin m(-2) after 28days of cultivation in a bench-scale TL-PSBR with a peridinin content in the dry biomass of 1% (w/w). Symbiodinium voratum may thus be a suitable source of peridinin for a diverse range of applications when grown in a twin-layer porous substrate bioreactor.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Langenbach, Dorothee UNSPECIFIED UNSPECIFIED UNSPECIFIED Melkonian, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-157875
DOI:	10.1007/s10811-018-1513-3
Journal or Publication Title:	J. Appl. Phycol.
Volume:	31
Number:	1
Page Range:	S. 21 - 29
Date:	2019
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-5176
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ALGAL CAROTENOIDS; HIGH LIGHT; MARINE; GROWTH; QUANTIFICATION; ASTAXANTHIN; CHLOROPHYLL; FUCOXANTHIN; PIGMENTS; CULTURE Multiple languages Biotechnology & Applied Microbiology; Marine & Freshwater Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15787