Universität zu Köln

Schlereth, Simona L., Karlstetter, Marcus, Hos, Deniz, Matthaei, Mario, Cursiefen, Claus and Heindl, Ludwig M. (2019). Detection of Pro- and Antiangiogenic Factors in the Human Sclera. Curr. Eye Res., 44 (2). S. 172 - 185. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1460-2202

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Abstract

Purpose: Avascular tissues can be used to identify antilymph- or antihemangiogenic factors. The human sclera-the outer covering layer of the eye, lacks lymphatic vessels and contains only a superficial network of blood vessels and was used here to identify endogenous antiangiogenic factors. Methods: Expression levels of a panel of 96 known pro- and antiangiogenic factors were analyzed in 12 scleral or conjunctival control samples from normal human donors using real-time PCR. In vitro, scleral homogenate was cocultured with blood- and lymphatic endothelial cells (BECs and LECs) and immunohistochemistry was performed of scleral fibroblasts and BECs. Results: Three antiangiogenic factors were significantly upregulated in the human sclera compared to the conjunctiva, including FBLN5 (fibulin 5), SERPINF1 (serpin peptidase inhibitor, clade F, member 1 = pigment epithelium derived factor) and TIMP2 (Tissue inhibitor of metalloproteinases 2). Six proangiogenic factors were significantly downregulated in the sclera, including FLT4 (Fms-related tyrosine kinase 4=VEGF-R3), HGF (hepatocyte growth factor), KIT (CD117 / c-kit), PROX1 (prospero homeobox 1), SEMA3F (semaphorin-3F) and TGFA (transforming growth factor alpha). In vitro, scleral homogenate inhibited the growth of both BECs and LECs. Immunohistochemistry labeling of three major antiangiogenic factors from scleral tissue confirmed TIMP3 and PEDF expression both in scleral fibroblasts and in blood endothelial cells, whereas TIMP2 was not detectable. Conclusion: Balancing anti- and proangiogenic factors actively regulates human scleral avascularity, inhibits endothelial cell growth in vitro, and thus may help maintaining the vascular privilege of the inner eye.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schlereth, Simona L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karlstetter, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED Hos, Deniz UNSPECIFIED UNSPECIFIED UNSPECIFIED Matthaei, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-157983
DOI:	10.1080/02713683.2018.1540704
Journal or Publication Title:	Curr. Eye Res.
Volume:	44
Number:	2
Page Range:	S. 172 - 185
Date:	2019
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	1460-2202
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TUMOR-ASSOCIATED LYMPHANGIOGENESIS; OCULAR IMMUNE PRIVILEGE; GENE-EXPRESSION; CORNEAL NEOVASCULARIZATION; MALIGNANT MELANOMAS; LYMPHATIC VESSELS; ENDOTHELIAL-CELLS; VEGF RECEPTOR-3; HUMAN FETAL; STEM-CELLS Multiple languages Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15798