Universität zu Köln

Uemura, Tomohiro ORCID: 0000-0001-7270-7986, Nakano, Ryohei Thomas ORCID: 0000-0001-5973-2300, Takagi, Junpei, Wang, Yiming ORCID: 0000-0003-0513-9039, Kramer, Katharina, Finkemeier, Iris ORCID: 0000-0002-8972-4026, Nakagami, Hirofumi ORCID: 0000-0003-2569-7062, Tsuda, Kenichi ORCID: 0000-0001-7074-0731, Ueda, Takashi ORCID: 0000-0002-5190-892X, Schulze-Lefert, Paul and Nakano, Akihiko (2019). A Golgi-Released Subpopulation of the Trans-Golgi Network Mediates Protein Secretion in Arabidopsis. Plant Physiol., 179 (2). S. 519 - 533. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

Spatiotemporal coordination of protein trafficking among organelles is essential for eukaryotic cells. The post-Golgi interface, including the trans-Golgi network (TGN), is a pivotal hub for multiple trafficking pathways. The Golgi-released independent TGN (GI-TGN) is a compartment described only in plant cells, and its cellular and physiological roles remain elusive. In Arabidopsis (Arabidopsis thaliana), the SYNTAXIN OF PLANTS (SYP) 4 group Qa-SNARE (soluble N-ethylmaleimide) membrane fusion proteins are shared components of TGN and GI-TGN and regulate secretory and vacuolar transport. Here we reveal that GI-TGNs mediate the transport of the R-SNARE VESICLE-ASSOCIATED MEMBRANE PROTEIN (VAMP) 721 to the plasma membrane. In interactions with a nonadapted powdery mildew pathogen, the SYP4 group of SNAREs is required for the dynamic relocation of VAMP721 to plant-fungus contact sites via GI-TGNs, thereby facilitating complex formation with its cognate SNARE partner PENETRATION1 to restrict pathogen entry. Furthermore, quantitative proteomic analysis of leaf apoplastic fluid revealed constitutive and pathogen-inducible secretion of cell wall-modification enzymes in a SYP4- and VAMP721-dependent manner. Hence, the GI-TGN acts as a transit compartment between the Golgi apparatus and the plasma membrane. We propose a model in which the GA-TGN matures into the GI-TGN and then into secretory vesicles by increasing the abundance of VAMP721-dependent secretory pathway components.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Uemura, Tomohiro UNSPECIFIED orcid.org/0000-0001-7270-7986 UNSPECIFIED Nakano, Ryohei Thomas UNSPECIFIED orcid.org/0000-0001-5973-2300 UNSPECIFIED Takagi, Junpei UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Yiming UNSPECIFIED orcid.org/0000-0003-0513-9039 UNSPECIFIED Kramer, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Finkemeier, Iris UNSPECIFIED orcid.org/0000-0002-8972-4026 UNSPECIFIED Nakagami, Hirofumi UNSPECIFIED orcid.org/0000-0003-2569-7062 UNSPECIFIED Tsuda, Kenichi UNSPECIFIED orcid.org/0000-0001-7074-0731 UNSPECIFIED Ueda, Takashi UNSPECIFIED orcid.org/0000-0002-5190-892X UNSPECIFIED Schulze-Lefert, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Nakano, Akihiko UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-158309
DOI:	10.1104/pp.18.01228
Journal or Publication Title:	Plant Physiol.
Volume:	179
Number:	2
Page Range:	S. 519 - 533
Date:	2019
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BROAD-SPECTRUM RESISTANCE; POWDERY MILDEW FUNGUS; DISEASE RESISTANCE; PEN1 SYNTAXIN; R PACKAGE; MEMBRANE; TRAFFICKING; IDENTIFICATION; CONSERVATION; TOMOGRAPHY Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15830