Universität zu Köln

Geisler, Florian, Coch, Richard A., Richardson, Christine ORCID: 0000-0003-2622-2261, Goldberg, Martin ORCID: 0000-0002-0610-4332, Denecke, Bernd, Bossinger, Olaf and Leube, Rudolf E. (2019). The intestinal intermediate filament network responds to and protects against microbial insults and toxins. Development, 146 (2). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9129

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Abstract

The enrichment of intermediate filaments in the apical cytoplasm of intestinal cells is evolutionarily conserved, forming a sheath that is anchored to apical junctions and positioned below the microvillar brush border, which suggests a protective intracellular barrier function. To test this, we used Caenorhabditis elegans, the intestinal cells of which are endowed with a particularly dense intermediate filament-rich layer that is referred to as the endotube. We found alterations in endotube structure and intermediate filament expression upon infection with nematicidal B. thuringiensis or treatment with its major pore-forming toxin crystal protein Cry5B. Endotube impairment due to defined genetic mutations of intermediate filaments and their regulators results in increased Cry5B sensitivity as evidenced by elevated larval arrest, prolonged time of larval development and reduced survival. Phenotype severity reflects the extent of endotube alterations and correlates with reduced rescue upon toxin removal. The results provide in vivo evidence for a major protective role of a properly configured intermediate filament network as an intracellular barrier in intestinal cells. This notion is further supported by increased sensitivity of endotube mutants to oxidative and osmotic stress.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Geisler, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Coch, Richard A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Richardson, Christine UNSPECIFIED orcid.org/0000-0003-2622-2261 UNSPECIFIED Goldberg, Martin UNSPECIFIED orcid.org/0000-0002-0610-4332 UNSPECIFIED Denecke, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Bossinger, Olaf UNSPECIFIED UNSPECIFIED UNSPECIFIED Leube, Rudolf E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-159153
DOI:	10.1242/dev.169482
Journal or Publication Title:	Development
Volume:	146
Number:	2
Date:	2019
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9129
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CAENORHABDITIS-ELEGANS; BARRIER FUNCTION; INNATE IMMUNITY; ORGANIZATION; EXPRESSION; KERATIN-8; INTEGRITY; VIRULENCE; PATHWAYS; PROTEINS Multiple languages Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15915