Universität zu Köln

Ramani, Anand, Mariappan, Aruljothi, Gottardo, Marco, Mandad, Sunit, Urlaub, Henning, Avidor-Reiss, Tomer ORCID: 0000-0003-0918-526X, Riparbelli, Maria, Callaini, Giuliano, Debec, Alain, Feederle, Regina and Gopalakrishnan, Jay (2018). Plk1/Polo Phosphorylates Sas-4 at the Onset of Mitosis for an Efficient Recruitment of Pericentriolar Material to Centrosomes. Cell Reports, 25 (13). S. 3618 - 3637. CAMBRIDGE: CELL PRESS. ISSN 2211-1247

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Abstract

Centrosomes are the major microtubule-organizing centers, consisting of centrioles surrounded by a pericentriolar material (PCM). Centrosomal PCM is spatiotemporally regulated to be minimal during interphase and expands as cells enter mitosis. It is unclear how PCM expansion is initiated at the onset of mitosis. Here, we identify that, in Drosophila, Plk1/Polo kinase phosphorylates the conserved centrosomal protein Sas-4 in vitro. This phosphorylation appears to occur at the onset of mitosis, enabling Sas-4's localization to expand outward from meiotic and mitotic centrosomes. The Plk1/Polo kinase site of Sas-4 is then required for an efficient recruitment of Cnn and gamma-tubulin, bona fide PCM proteins that are essential for PCM expansion and centrosome maturation. Point mutations at Plk1/Polo sites of Sas-4 affect neither centrosome structure nor centriole duplication but specifically reduce the affinity to bind Cnn and gamma-tubulin. These observations identify Plk1/Polo kinase regulation of Sas-4 as essential for efficient PCM expansion.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ramani, Anand UNSPECIFIED UNSPECIFIED UNSPECIFIED Mariappan, Aruljothi UNSPECIFIED UNSPECIFIED UNSPECIFIED Gottardo, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Mandad, Sunit UNSPECIFIED UNSPECIFIED UNSPECIFIED Urlaub, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Avidor-Reiss, Tomer UNSPECIFIED orcid.org/0000-0003-0918-526X UNSPECIFIED Riparbelli, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Callaini, Giuliano UNSPECIFIED UNSPECIFIED UNSPECIFIED Debec, Alain UNSPECIFIED UNSPECIFIED UNSPECIFIED Feederle, Regina UNSPECIFIED UNSPECIFIED UNSPECIFIED Gopalakrishnan, Jay UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-161736
DOI:	10.1016/j.celrep.2018.11.102
Journal or Publication Title:	Cell Reports
Volume:	25
Number:	13
Page Range:	S. 3618 - 3637
Date:	2018
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2211-1247
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DAUGHTER CENTRIOLES; DROSOPHILA SPD-2; GAMMA-TUBULIN; POLO; SIZE; ASTERLESS; KINASE; CPAP; PCM Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16173