Universität zu Köln

Strubl, Sebastian, Schubert, Uwe, Kuehnle, Andrea, Rebl, Alexander ORCID: 0000-0002-2918-7433, Ahmadvand, Negah, Fischer, Silvia, Preissner, Klaus T. and Galuska, Sebastian P. (2018). Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro. Cell Biosci., 8. LONDON: BMC. ISSN 2045-3701

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Abstract

BackgroundSialic acids represent common terminal residues on numerous mammalian glycoconjugates, thereby influencing e.g. lumen formation in developing blood vessels. Interestingly, besides monosialylated also polysialylated glycoconjugates are produced by endothelial cells. Polysialic acid (polySia) is formed in several organs during embryonal and postnatal development influencing, for instance, cell migration processes. Furthermore, the function of cytokines like basic fibroblast growth factor (bFGF) is modulated by polySia.ResultsIn this study, we demonstrated that human umbilical vein endothelial cells (HUVEC) also secrete polysialylated glycoconjugates. Furthermore, an interaction between polySia and vascular endothelial growth factor (VEGF) was observed. VEGF modulates like bFGF the migration of HUVEC. Since both growth factors interact with polySia, we examined, if polySia modulates the migration of HUVEC. To this end scratch assays were performed showing that the migration of HUVEC is stimulated, when polySia was degraded.ConclusionsSince polySia can interact with bFGF as well as VEGF and the degradation of polySia resulted in an increased cell migration capacity in the applied scratch assay, we propose that polySia may trap these growth factors influencing their biological activity. Thus, polySia might also contribute to the fine regulation of physiological processes in endothelial cells.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Strubl, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schubert, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuehnle, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Rebl, Alexander UNSPECIFIED orcid.org/0000-0002-2918-7433 UNSPECIFIED Ahmadvand, Negah UNSPECIFIED UNSPECIFIED UNSPECIFIED Fischer, Silvia UNSPECIFIED UNSPECIFIED UNSPECIFIED Preissner, Klaus T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Galuska, Sebastian P. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-162194
DOI:	10.1186/s13578-018-0262-y
Journal or Publication Title:	Cell Biosci.
Volume:	8
Date:	2018
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	2045-3701
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FIBROBLAST-GROWTH-FACTOR; ADHESION MOLECULE NCAM; SIALIC ACIDS; POSTTRANSLATIONAL MODIFICATION; NEUROTROPHIC FACTOR; MIGRATION; POLYSIALYLATION; BINDING; SYSTEM; POLYSIALYLTRANSFERASE Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16219