Universität zu Köln

Klimpel, Annika and Neundorf, Ines ORCID: 0000-0001-6450-3991 (2018). Bifunctional peptide hybrids targeting the matrix of mitochondria. J. Control. Release, 291. S. 147 - 157. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1873-4995

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Abstract

The mitochondrial organelle is associated with many diseases, including diabetes, age-related neuro-degenerative diseases and cancer. Therefore, the effective delivery of drug molecules to mitochondria became increasingly important during the past years. Within this work, we designed and analyzed bifunctional hybrid peptides comprised of a mitochondrial targeting sequence (MTS) attached to a cell-penetrating peptide (CPP). Our results demonstrate that choice of the MTS must be carefully undertaken, since not every MTS that was selected was comparably capable to target mitochondria. In addition, we highlight the use of the CPP sC18 as necessary part of the hybrid construct, inducing not only cellular uptake, but likewise supporting sub-organelle uptake into the mitochondrial matrix. The herein designed cell-permeable mitochondrial targeting peptide was furthermore proven to enhance the intracellular uptake of the cytostatic drug chlorambucil, making it a powerful candidate for further studies in this important field.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klimpel, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Neundorf, Ines UNSPECIFIED orcid.org/0000-0001-6450-3991 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-162251
DOI:	10.1016/j.jconrel.2018.10.029
Journal or Publication Title:	J. Control. Release
Volume:	291
Page Range:	S. 147 - 157
Date:	2018
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1873-4995
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-PENETRATING PEPTIDE; CANCER; DRUG; SEQUENCE; IMPORT; CLEAVAGE; DELIVERY; DISEASE; PROTEIN; FUSION Multiple languages Chemistry, Multidisciplinary; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16225