Universität zu Köln

Huelsemann, Frank, Gougoulidis, Vassilios, Schertel, Thomas, Fusshoeller, Gregor, Flenker, Ulrich, Piper, Thomas and Thevis, Mario (2018). Case Study: Atypical delta C-13 values of urinary norandrosterone. Drug Test. Anal., 10 (11-12). S. 1728 - 1734. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

Isotope ratio mass spectrometry (IRMS) has been established in doping control analysis to identify the endogenous or exogenous origin of a variety of steroidal analytes including the 19-norsteroid metabolite norandrosterone (NorA). NorA can be found naturally in human urine in trace amounts due to endogenous demethylation or in situ microbial degradation. The administration of nortestosterone (nandrolone) or different prohormones results in the excretion of urinary NorA. Usually, this can be detected by IRMS due to differing delta C-13 values of synthetic 19-norsteroids compared to endogenous reference compounds. The consumption of uncastrated pig edible parts like offal or even meat may also lead to a urinary excretion of NorA. In order to determine the delta C-13 values of such a scenario, urine samples collected after consumption of a wild-boar-testicle meal were analyzed. IRMS revealed highly enriched delta C-13 values for urinary NorA, which could be related to the completely corn-based nutrition of the animal. Isotopic analysis of the boar's bristles demonstrated a dietary change from C-3-based forage, probably in winter and spring, to a C-4-based diet in the last weeks to months prior to death. These results supported the interpretation of an atypical test result of a Central European athlete's doping control sample with delta C-13 values for NorA of -18 parts per thousand, most probably caused by the consumption of a wild boar ragout. As stated before, athletes should be fully aware of the risk that consumption of wild boar may result in atypical or even adverse analytical findings in sports drug testing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Huelsemann, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Gougoulidis, Vassilios UNSPECIFIED UNSPECIFIED UNSPECIFIED Schertel, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Fusshoeller, Gregor UNSPECIFIED UNSPECIFIED UNSPECIFIED Flenker, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Piper, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-166571
DOI:	10.1002/dta.2498
Journal or Publication Title:	Drug Test. Anal.
Volume:	10
Number:	11-12
Page Range:	S. 1728 - 1734
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EDIBLE TISSUE CONSUMPTION; STABLE-ISOTOPE RATIOS; SUS-SCROFA L.; NANDROLONE METABOLITES; DOPING CONTROL; CROP DAMAGE; CARBON; 19-NORANDROSTERONE; NITROGEN; STEROIDS Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16657