Goergens, Christian, Guddat, Sven, Thomas, Andreas ORCID: 0000-0003-1199-0743 and Thevis, Mario (2018). Recent improvements in sports drug testing concerning the initial testing for peptidic drugs (< 2 kDa) - sample preparation, mass spectrometric detection, and data review. Drug Test. Anal., 10 (11-12). S. 1755 - 1761. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

In this work, a novel initial testing assay based on liquid chromatography-mass spectrometry is presented, enabling the detection of peptidic drugs and drug candidates (< 2 kDa) prohibited in sports. The assay covers representatives and metabolites of gonadotropin releasing hormone and its analogs (GnRHs), growth hormone secretagogues (GHS), growth hormone releasing peptides (GHRPs), and the Vasopressin-analog Desmopressin. The general objective of this work was to reduce sample preparation efforts to a minimum while preserving highest possible sensitivity and specificity of the assay, demonstrating limits of detection between 50 and 200 pg/mL. Here, a dilute-and-inject strategy provides the simplest conceivable sample preparation procedure. Furthermore, the combination of well-established strategies for the determination of peptides, such as two-dimensional liquid chromatography, dimethyl sulfoxide (DMSO)-assisted electrospray ionization, high resolution mass spectrometric detection and a tailored reporter template, which facilitates data review enormously, provides a high-throughput initial testing assay for lower molecular mass peptidic and peptide-related analytes.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Goergens, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Guddat, SvenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thomas, AndreasUNSPECIFIEDorcid.org/0000-0003-1199-0743UNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-166586
DOI: 10.1002/dta.2503
Journal or Publication Title: Drug Test. Anal.
Volume: 10
Number: 11-12
Page Range: S. 1755 - 1761
Date: 2018
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1942-7611
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
DIRECT URINE INJECTION; LIQUID-CHROMATOGRAPHYMultiple languages
Biochemical Research Methods; Chemistry, Analytical; Pharmacology & PharmacyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/16658

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