Thomas, Andreas ORCID: 0000-0003-1199-0743 and Thevis, Mario (2018). Analysis of insulin and insulin analogs from dried blood spots by means of liquid chromatography-high resolution mass spectrometry. Drug Test. Anal., 10 (11-12). S. 1761 - 1769. HOBOKEN: WILEY. ISSN 1942-7611

Full text not available from this repository.

Abstract

While dried blood spot (DBS) analysis concerning low molecular mass molecules has become more and more established in various fields of analytical chemistry, the utility of DBS in determining peptides and proteins from DBS is yet comparably limited. In consideration of the fact that the apparent benefits of DBS sampling are similar for analytes of lower and higher molecular mass, dedicated (non-generic) sample preparation procedures are required that meet the needs for detecting peptidic drugs and hormones in DBS. The analysis of insulin and its synthetic analogs by mass spectrometry has received increased attention in several fields such as doping controls, forensics, and drug metabolism and pharmacokinetics studies. Hence, a strategy facilitating the analysis of insulin and its synthetic or animal analogs (human, Lispro, Aspart, Glulisine, Glargine, Detemir, Tresiba, and porcine and bovine insulin) from DBS was developed. The successful analysis of these substances at physiologically relevant concentrations was realized after ultrasonication-assisted extraction, immunoaffinity purification, and liquid chromatographic separation followed by high resolution mass spectrometric detection (with or without ion mobility). Assay validation demonstrated adequate sensitivity (LOD 0.5 ng/mL for most insulins), as well as precise (< 25%) and reproducible results for all included target insulins. Additionally, proof-of-principle data were obtained by the analysis of DBS samples obtained from healthy volunteers in non-fasting state as well as a sample from a diabetic volunteer treated with the fast acting analog insulin Aspart.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Thomas, AndreasUNSPECIFIEDorcid.org/0000-0003-1199-0743UNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-166594
DOI: 10.1002/dta.2518
Journal or Publication Title: Drug Test. Anal.
Volume: 10
Number: 11-12
Page Range: S. 1761 - 1769
Date: 2018
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1942-7611
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GROWTH-HORMONE; LC-MS/MS; QUANTIFICATION; DBS; QUANTITATION; ASSAY; KDAMultiple languages
Biochemical Research Methods; Chemistry, Analytical; Pharmacology & PharmacyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/16659

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item