Universität zu Köln

Du, Xinxin ORCID: 0000-0002-5128-7158, Klaschik, Kristina, Mallmann, Peter, Isachenko, Evgenia, Rahimi, Gohar, Zhao, Yue, Bruns, Christiane and Isachenko, Vladimir (2018). An Experimental Model of Breast Cancer Cells: Informative Protocol for In Vitro Culture. Anticancer Res., 38 (11). S. 6237 - 6246. ATHENS: INT INST ANTICANCER RESEARCH. ISSN 1791-7530

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Abstract

Background: Cryopreservation of ovarian tissue for patients with cancer comes with the concern for the existence of cancer cells in the grafts. Data about cancer cell viability after cryopreservation are currently lacking. For the experimental study of cryo-stability of cancer cells, different protocols of in vitro culture of various cell lines are used. The existing protocols are not able to mimic the effectiveness of in vivo/in situ development of cancer cells. This study aimed to test the new protocol for in vitro culture of breast cancer cells. Materials and Methods: ZR-75-1 and MDA-MB-231 cells were in vitro-cultured in standard [RPMI 1640 and Dulbecco's modified Eagle's medium (DMEM), respectively] and experimental (AIM V) medium. Cell phenotypes were tested by CCK-8 cell proliferation measurement, wound-healing assay, transmembrane cell migration and invasion assay and immunofluorescent staining. A 10-day in vitro culture without cell passaging was conducted on both cell lines in culture medium to observe the generation of a cell layer as a model of solid tumor tissue. The density of the cell layers was revealed by immunofluorescent staining. Results: AIM V significantly promoted continued proliferation of both cell lines. Cell motility of ZR-75-1 cells was increased considerably more in AIM V than in RPMI-1640. For MDA-MB-231 cells cultured in AIM V and DMEM, no significant differences in mobility and invasion were observed. Both cell lines maintained in AIM V medium generated a cell layer on day 7 and formed a compact structure on day 10 of in vitro culture. Conclusion: In vitro culture of ZR-75-1 and MDA-MB-231 cells in AIM V medium is more informative than culture in standard medium. The described protocol provides a means for the formation of compact structures from in vitro-cultured cancer cells as a model of solid tumor tissue.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Du, Xinxin UNSPECIFIED orcid.org/0000-0002-5128-7158 UNSPECIFIED Klaschik, Kristina UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhao, Yue UNSPECIFIED UNSPECIFIED UNSPECIFIED Bruns, Christiane UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Vladimir UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-167699
DOI:	10.21873/anticanres.12979
Journal or Publication Title:	Anticancer Res.
Volume:	38
Number:	11
Page Range:	S. 6237 - 6246
Date:	2018
Publisher:	INT INST ANTICANCER RESEARCH
Place of Publication:	ATHENS
ISSN:	1791-7530
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRYOPRESERVED OVARIAN TISSUE; TUMOR; CORTEX; TRANSPLANTATION; PRESERVATION; HEPATOCYTES; PREGNANCY; LEUKEMIA Multiple languages Oncology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16769