Universität zu Köln

Raspa, Marcello, Mahabir, Esther, Paoletti, Renata, Protti, Michele ORCID: 0000-0001-9310-4957, Mercolini, Laura, Schiller, Petra and Scavizzi, Ferdinando (2018). Effects of oral D-aspartate on sperm quality in B6N mice. Theriogenology, 121. S. 53 - 62. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1879-3231

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Abstract

The goal of this study was to investigate the effects of oral administration of D-aspartate (D-Asp) to sexually immature male C57BL/6NTacCnrm (B6N) mice on the in vitro fertilisation (IVF) rate with cryopreserved-thawed spermatozoa and on cryopreserved sperm quality as well as on LH, epitestosterone, and testosterone levels. Males were treated at 7 weeks of age with a dose of 20 mM sodium D-aspartate in drinking water for 1.3, 2, 4 or 6 weeks so that they were 8.3, 9, 11 or 13 weeks of age, respectively, at the end of the study. The timepoints for controls were week 0 (start of experiment), 1.3, 2, 4 or 6, whereby mice received no D-Asp. At each timepoint, spermatozoa were cryopreserved for IVF and testes as well as sera were frozen for hormone level measurement. After 2 and 4 weeks of treatment, the IVF rate was significantly higher in the D-Asp group than in the controls (64% vs. 44% and 52% vs. 38%, respectively). Spermatozoa from D-Asp-treated males showed lower morphological abnormalities than their control counterparts. After 2 and especially after 4 weeks of treatment, the hormone levels in sera and testes and the total and progressive motility of the spermatozoa were higher in D-Asp-treated males. Although we did not elucidate the full mechanism leading to an improved IVF rate with spermatozoa from D-Asp-treated males lower morphological abnormalities and increased motility contribute to this observation. Importantly, D-Asp significantly improved the IVF rates as early as 2 weeks after treatment when mice were only 9 weeks old. This implies that sperm can be efficiently cryopreserved from younger males, compared to 13-weeks-old males, which are usually used for sperm cryopreservation. This is of relevance when genetic alterations cause premature death in males as well as high severity levels in older mice and aids in better resource management. (C) 2018 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Raspa, Marcello UNSPECIFIED UNSPECIFIED UNSPECIFIED Mahabir, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Paoletti, Renata UNSPECIFIED UNSPECIFIED UNSPECIFIED Protti, Michele UNSPECIFIED orcid.org/0000-0001-9310-4957 UNSPECIFIED Mercolini, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiller, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Scavizzi, Ferdinando UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-168569
DOI:	10.1016/j.theriogenology.2018.07.040
Journal or Publication Title:	Theriogenology
Volume:	121
Page Range:	S. 53 - 62
Date:	2018
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1879-3231
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SUPPORT EMBRYO DEVELOPMENT; IN-VITRO FERTILIZATION; PROTECTS BULL SPERM; MOUSE SPERM; EPIDIDYMAL MATURATION; HEAD ABNORMALITIES; RAT TESTIS; ACID; SPERMATOZOA; SPERMATOGENESIS Multiple languages Reproductive Biology; Veterinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16856