Universität zu Köln

Albanna, Walid ORCID: 0000-0001-9986-8739, Kotliar, Konstantin, Lueke, Jan Niklas, Alpdogan, Serdar ORCID: 0000-0002-5188-9925, Conzen, Catharina, Lindauer, Ute, Clusmann, Hans, Hescheler, Juergen, Vilser, Walthard, Schneider, Toni and Schubert, Gerrit Alexander ORCID: 0000-0001-9135-6042 (2018). Non-invasive evaluation of neurovascular coupling in the murine retina by dynamic retinal vessel analysis. PLoS One, 13 (10). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Background Impairment of neurovascular coupling (NVC) was recently reported in the context of subarachnoid hemorrhage and may correlate with disease severity and outcome. However, previous techniques to evaluate NVC required invasive procedures. Retinal vessels may represent an alternative option for non-invasive assessment of NVC. Methods A prototype of an adapted retinal vessel analyzer was used to assess retinal vessel diameter in mice. Dynamic vessel analysis (DVA) included an application of monochromatic flicker light impulses in predefined frequencies for evaluating NVC. All retinae were harvested after DVA and electroretinograms were performed. Results A total of 104 retinal scans were conducted in 21 male mice (90 scans). Quantitative arterial recordings were feasible only in a minority of animals, showing an emphasized reaction to flicker light impulses (8 mice; 14 scans). A characteristic venous response to flicker light, however, could observed in the majority of animals. Repeated measurements resulted in a significant decrease of baseline venous diameter (7 mice; 7 scans, p < 0.05). Ex-vivo electroretinograms, performed after in-vivo DVA, demonstrated a significant reduction of trans-retinal signaling in animals with repeated DVA (n = 6, p < 0.001). Conclusions To the best of our knowledge, this is the first non-invasive study assessing murine retinal vessel response to flicker light with characteristic changes in NVC. The imaging system can be used for basic research and enables the investigation of retinal vessel dimension and function in control mice and genetically modified animals.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Albanna, Walid UNSPECIFIED orcid.org/0000-0001-9986-8739 UNSPECIFIED Kotliar, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Lueke, Jan Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Alpdogan, Serdar UNSPECIFIED orcid.org/0000-0002-5188-9925 UNSPECIFIED Conzen, Catharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Lindauer, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Clusmann, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Vilser, Walthard UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Toni UNSPECIFIED UNSPECIFIED UNSPECIFIED Schubert, Gerrit Alexander UNSPECIFIED orcid.org/0000-0001-9135-6042 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-169592
DOI:	10.1371/journal.pone.0204689
Journal or Publication Title:	PLoS One
Volume:	13
Number:	10
Date:	2018
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Medicine > Physiologie und Pathophysiologie > Institut für Neurophysiologie
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SUBARACHNOID HEMORRHAGE; CEREBRAL AUTOREGULATION; FLICKERING LIGHT; BLOOD-FLOW; AGE; VASODILATION; DIAMETER; RAT Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16959