Universität zu Köln

Bhatia, Chitra, Pandey, Ashutosh, Gaddam, Subhash Reddy, Hoecker, Ute ORCID: 0000-0002-5636-9777 and Trivedi, Prabodh Kumar (2018). Low Temperature-Enhanced Flavonol Synthesis Requires Light-Associated Regulatory Components in Arabidopsis thaliana. Plant Cell Physiol., 59 (10). S. 2099 - 2113. OXFORD: OXFORD UNIV PRESS. ISSN 1471-9053

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Abstract

Plants are continuously exposed to a myriad of stresses, which lead to the formation of secondary metabolites including flavonoids. Studies suggest that low temperature exposure leads to enhanced flavonoid accumulation in Arabidopsis thaliana. In addition, flavonoid biosynthesis is regulated by light through various regulatory factors. Therefore, plants may possess the capability to integrate light and low temperature signals for survival under freezing conditions. However, the detailed molecular mechanism and the regulatory factors associated with light- and low temperature-responsive flavonoid biosynthesis remain largely unknown. Here, we report a strict requirement for light for the low temperature-enhanced flavonol biosynthesis. Low temperature-induced expression of biosynthetic genes as well as flavonol accumulation was hampered in ELONGATED HYPOCOTYL (hy5) and myb11myb111myb12 triple mutants as compared with the wild type in Arabidopsis. Overexpression of AtHY5 in the hy5 mutant restored induction of gene expression and flavonol accumulation in response to low temperature in light. Metabolite and gene expression analysis also suggests a negative role for CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) in accumulation of flavonols in response to low temperature. Overexpression of AtMYB12 enhanced accumulation of flavonols under low temperature in a light- dependent manner. Together, our analysis suggests the requirement for HY5 and flavonol-specific MYB regulatory factors for low temperature-induced flavonol synthesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bhatia, Chitra UNSPECIFIED UNSPECIFIED UNSPECIFIED Pandey, Ashutosh UNSPECIFIED UNSPECIFIED UNSPECIFIED Gaddam, Subhash Reddy UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Ute UNSPECIFIED orcid.org/0000-0002-5636-9777 UNSPECIFIED Trivedi, Prabodh Kumar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-170473
DOI:	10.1093/pcp/pcy132
Journal or Publication Title:	Plant Cell Physiol.
Volume:	59
Number:	10
Page Range:	S. 2099 - 2113
Date:	2018
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1471-9053
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ANTHOCYANIN BIOSYNTHETIC-PATHWAY; TRANSCRIPTION FACTORS; COLD-ACCLIMATION; GENE-EXPRESSION; FREEZING TOLERANCE; AUXIN TRANSPORT; HY5; ACCUMULATION; PROTEIN; ACTIVATION Multiple languages Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17047