Universität zu Köln

Korkmaz, Y., Roggendorf, H. C., Siefer, O. G., Seehawer, J., Imhof, T., Plomann, M., Bloch, W., Friebe, A. and Huebbers, C. U. (2018). Downregulation of the (1)- and (1)-subunit of sGC in Arterial Smooth Muscle Cells of OPSCC Is HPV-Independent. J. Dent. Res., 97 (11). S. 1214 - 1222. THOUSAND OAKS: SAGE PUBLICATIONS INC. ISSN 1544-0591

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Abstract

The nitric oxide (NO)-sensitive soluble guanylyl cyclase (sGC) is a heterodimeric enzyme with an and subunit. NO binds to heme of the (1)-subunit of sGC, activates the enzyme in the reduced heme iron state in vascular smooth muscle cells (VSMCs), and generates cGMP-inducing vasodilatation and suppression of VSMC proliferation. In the complex tumor milieu with higher levels of reactive oxygen species (ROS), sGC heme iron may become oxidized and insensitive to NO. To change sGC from an NO-insensitive to NO-sensitive state or NO-independent manner, protein expression of sGC in VSMC is required. Whether sGC(11) exists at the protein level in arterial VSMCs of oropharyngeal squamous cell carcinoma (OPSCC) is unknown. In addition, whether differences in the genetic profile between human papillomavirus (HPV)-positive and HPV-negative OPSCC contributes to the regulation of sGC(11) is unclear. Therefore, we compared the effects of HPV-positive and HPV-negative OPSCC on the expression of sGC(11) in arterial VSMCs from tumor-free and tumor-containing regions of human tissue sections using quantitative immunohistochemistry. In comparison to the tumor-free region, we found a decrease in expression of both (1)- and (1)-subunits in the arterial VSMC layer of the tumor-containing areas. The OPSCC-induced significant downregulation of the (1)- and (1)-subunits of sGC in arterial VSMC was HPV-independent. We conclude that the response of sGC to NO in tumor arterial VSMCs may be impaired by oxidation of the heme of the (1)-subunit, and thus, (1)- and (1)-subunits of sGC could be targeted to degradation under oxidative stress in OPSCC in an HPV-independent manner. The degradation of sGC(11) in VSMCs may result in increased proliferation of VSMCs, promoting tumor arteriogenesis in OPSCC. This can be interrupted by preserving the active heterodimer sGC(11) in arterial VSMCs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Korkmaz, Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roggendorf, H. C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Siefer, O. G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Seehawer, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Imhof, T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Plomann, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bloch, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Friebe, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huebbers, C. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-171791
DOI:	10.1177/0022034518774531
Journal or Publication Title:	J. Dent. Res.
Volume:	97
Number:	11
Page Range:	S. 1214 - 1222
Date:	2018
Publisher:	SAGE PUBLICATIONS INC
Place of Publication:	THOUSAND OAKS
ISSN:	1544-0591
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SOLUBLE GUANYLATE-CYCLASE; NITRIC-OXIDE; HUMAN-PAPILLOMAVIRUS; NECK-CANCER; CYCLIC-GMP; HEAD; CARCINOMA; ANGIOGENESIS; STIMULATORS; CHEMOKINES Multiple languages Dentistry, Oral Surgery & Medicine Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17179