Universität zu Köln

Xue, Li ORCID: 0000-0002-9367-0155, Klinnawee, Lompong ORCID: 0000-0001-7388-3148, Zhou, Yue, Saridis, Georgios ORCID: 0000-0001-8781-6886, Vijayakumar, Vinod ORCID: 0000-0002-4923-5551, Brands, Mathias ORCID: 0000-0001-6548-1448, Doermann, Peter, Gigolashvili, Tamara ORCID: 0000-0002-0416-4796, Turck, Franziska ORCID: 0000-0002-4982-5949 and Bucher, Marcel (2018). AP2 transcription factor CBX1 with a specific function in symbiotic exchange of nutrients in mycorrhizal Lotus japonicus. Proc. Natl. Acad. Sci. U. S. A., 115 (39). S. E9239 - 8. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

Full text not available from this repository.

Abstract

The arbuscular mycorrhizal (AM) symbiosis, a widespread mutualistic association between land plants and fungi, depends on reciprocal exchange of phosphorus driven by proton-coupled phosphate uptake into host plants and carbon supplied to AM fungi by host-dependent sugar and lipid biosynthesis. The molecular mechanisms and cis-regulatory modules underlying the control of phosphate uptake and de novo fatty acid synthesis in AM symbiosis are poorly understood. Here, we show that the AP2 family transcription factor CTTC MOTIF-BINDING TRANSCRIPTION FACTOR1 (CBX1), a WRINKLED1 (WRI1) homolog, directly binds the evolutionary conserved CTTC motif that is enriched in mycorrhiza-regulated genes and activates Lotus japonicus phosphate transporter 4 (LjPT4) in vivo and in vitro. Moreover, the mycorrhiza-inducible gene encoding H+-ATPase (LjHA1), implicated in energizing nutrient uptake at the symbiotic interface across the periarbuscular membrane, is coregulated with LjPT4 by CBX1. Accordingly, CBX1-defective mutants show reduced mycorrhizal colonization. Furthermore, genome-wide-binding profiles, DNA-binding studies, and heterologous expression reveal additional binding of CBX1 to AW box, the consensus DNA-binding motif for WRI1, that is enriched in promoters of glycolysis and fatty acid biosynthesis genes. We show that CBX1 activates expression of lipid metabolic genes including glycerol-3-phosphate acyltransferase RAM2 implicated in acylglycerol biosynthesis. Our finding defines the role of CBX1 as a regulator of host genes involved in phosphate uptake and lipid synthesis through binding to the CTTC/AW molecular module, and supports a model underlying bidirectional exchange of phosphorus and carbon, a fundamental trait in the mutualistic AM symbiosis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Xue, Li UNSPECIFIED orcid.org/0000-0002-9367-0155 UNSPECIFIED Klinnawee, Lompong UNSPECIFIED orcid.org/0000-0001-7388-3148 UNSPECIFIED Zhou, Yue UNSPECIFIED UNSPECIFIED UNSPECIFIED Saridis, Georgios UNSPECIFIED orcid.org/0000-0001-8781-6886 UNSPECIFIED Vijayakumar, Vinod UNSPECIFIED orcid.org/0000-0002-4923-5551 UNSPECIFIED Brands, Mathias UNSPECIFIED orcid.org/0000-0001-6548-1448 UNSPECIFIED Doermann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Gigolashvili, Tamara UNSPECIFIED orcid.org/0000-0002-0416-4796 UNSPECIFIED Turck, Franziska UNSPECIFIED orcid.org/0000-0002-4982-5949 UNSPECIFIED Bucher, Marcel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-172535
DOI:	10.1073/pnas.1812275115
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	115
Number:	39
Page Range:	S. E9239 - 8
Date:	2018
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARBUSCULAR-MYCORRHIZAL; PHOSPHATE TRANSPORTER; MEDICAGO-TRUNCATULA; GENE FAMILY; H+-ATPASE; RHIZOPHAGUS-IRREGULARIS; PHT1 FAMILY; BIOSYNTHESIS; ARABIDOPSIS; RICE Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17253