Universität zu Köln

Pfau, Thomas, Christian, Nils, Masakapalli, Shyam K., Sweetlove, Lee J., Poolman, Mark G. and Ebenhoeh, Oliver ORCID: 0000-0002-7229-7398 (2018). The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling. Sci Rep, 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Genome-scale metabolic network models can be used for various analyses including the prediction of metabolic responses to changes in the environment. Legumes are well known for their rhizobial symbiosis that introduces nitrogen into the global nutrient cycle. Here, we describe a fully compartmentalised, mass and charge-balanced, genome-scale model of the clover Medicago truncatula, which has been adopted as a model organism for legumes. We employed flux balance analysis to demonstrate that the network is capable of producing biomass components in experimentally observed proportions, during day and night. By connecting the plant model to a model of its rhizobial symbiont, Sinorhizobium meliloti, we were able to investigate the effects of the symbiosis on metabolic fluxes and plant growth and could demonstrate how oxygen availability influences metabolic exchanges between plant and symbiont, thus elucidating potential benefits of inter organism amino acid cycling. We thus provide a modelling framework, in which the interlinked metabolism of plants and nodules can be studied from a theoretical perspective.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pfau, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Christian, Nils UNSPECIFIED UNSPECIFIED UNSPECIFIED Masakapalli, Shyam K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sweetlove, Lee J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Poolman, Mark G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ebenhoeh, Oliver UNSPECIFIED orcid.org/0000-0002-7229-7398 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-176156
DOI:	10.1038/s41598-018-30884-x
Journal or Publication Title:	Sci Rep
Volume:	8
Date:	2018
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FLUX BALANCE ANALYSIS; LEGUME MEDICAGO-TRUNCATULA; HETEROTROPHIC ARABIDOPSIS CELLS; SINORHIZOBIUM-MELILOTI; NODULE DEVELOPMENT; STRESS CONDITIONS; ROOT-NODULE; NETWORK; RECONSTRUCTION; BACTEROIDS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17615