Universität zu Köln

Simao, Daniel ORCID: 0000-0002-7532-8081, Silva, Marta M., Terrasso, Ana P., Arez, Francisca, Sousa, Marcos F. Q., Mehrjardi, Narges Z., Saric, Tomo, Gomes-Alves, Patricia ORCID: 0000-0001-7245-6785, Raimundo, Nuno ORCID: 0000-0002-5988-9129, Alves, Paula M. and Brito, Catarina (2018). Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3D Differentiation. Stem Cell Rep., 11 (2). S. 552 - 565. CAMBRIDGE: CELL PRESS. ISSN 2213-6711

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Abstract

Brain microenvironment plays an important role in neurodevelopment and pathology, where the extracellular matrix (ECM) and soluble factors modulate multiple cellular processes. Neural cell culture typically relies on heterologous matrices poorly resembling brain ECM. Here, we employed neurospheroids to address microenvironment remodeling during neural differentiation of human stem cells, without the confounding effects of exogenous matrices. Proteome and transcriptome dynamics revealed significant changes at cell membrane and ECM during 3D differentiation, diverging significantly from the 2D differentiation. Structural proteoglycans typical of brain ECM were enriched during 3D differentiation, in contrast to basement membrane constituents in 2D. Moreover, higher expression of synaptic and ion transport machinery was observed in 3D cultures, suggesting higher neuronal maturation in neurospheroids. This work demonstrates that 3D neural differentiation as neurospheroids promotes the expression of cellular and extracellular features found in neural tissue, highlighting its value to address molecular defects in cell-ECM interactions associated with neurological disorders.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Simao, Daniel UNSPECIFIED orcid.org/0000-0002-7532-8081 UNSPECIFIED Silva, Marta M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Terrasso, Ana P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Arez, Francisca UNSPECIFIED UNSPECIFIED UNSPECIFIED Sousa, Marcos F. Q. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mehrjardi, Narges Z. UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED UNSPECIFIED UNSPECIFIED Gomes-Alves, Patricia UNSPECIFIED orcid.org/0000-0001-7245-6785 UNSPECIFIED Raimundo, Nuno UNSPECIFIED orcid.org/0000-0002-5988-9129 UNSPECIFIED Alves, Paula M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brito, Catarina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-176503
DOI:	10.1016/j.stemcr.2018.06.020
Journal or Publication Title:	Stem Cell Rep.
Volume:	11
Number:	2
Page Range:	S. 552 - 565
Date:	2018
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2213-6711
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PLURIPOTENT STEM-CELLS; CENTRAL-NERVOUS-SYSTEM; EXTRACELLULAR-MATRIX; CEREBRAL ORGANOIDS; BRAIN; ADHESION; NEURONS; METALLOPROTEINASES; PROTEOGLYCANS; SPECIFICATION Multiple languages Cell & Tissue Engineering; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17650