Universität zu Köln

Zhang, Xinxin, Koester, Philipp, Schluecking, Kathrin, Balcerowicz, Daria, Hashimoto, Kenji, Kuchitsu, Kazuyuki ORCID: 0000-0001-8854-0375, Vissenberg, Kris ORCID: 0000-0003-0292-2095 and Kudla, Joerg (2018). CBL1-CIPK26-mediated phosphorylation enhances activity of the NADPH oxidase RBOHC, but is dispensable for root hair growth. FEBS Lett., 592 (15). S. 2582 - 2594. HOBOKEN: WILEY. ISSN 1873-3468

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Abstract

Root hairs (RH) are tip growing polarized cells aiding the uptake of nutrients and water into plants. RH differentiation involves the interplay of various hormones and second messengers. Tightly regulated production of reactive oxygen species by the NADPH oxidase RBOHC crucially functions in RH differentiation and Ca2+-dependent phosphorylation has been implemented in these processes. However, the kinases regulating RBOHC remained enigmatic. Here we identify CBL1-CIPK26 Ca2+ sensor-kinase complexes as modulators of RBOHC activity. Combined genetic, cell biological and biochemical analyses reveal synergistic function of CIPK26-mediated phosphorylation and Ca2+ binding for RBOHC activation. Complementation of rhohC mutant RH phenotypes by a S318/322 phosphorylation deficient RBOHC version suggests flexible and alternating phosphorylation patterns as mechanism fine-tuning ROS production in RH development.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhang, Xinxin UNSPECIFIED UNSPECIFIED UNSPECIFIED Koester, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Schluecking, Kathrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Balcerowicz, Daria UNSPECIFIED UNSPECIFIED UNSPECIFIED Hashimoto, Kenji UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuchitsu, Kazuyuki UNSPECIFIED orcid.org/0000-0001-8854-0375 UNSPECIFIED Vissenberg, Kris UNSPECIFIED orcid.org/0000-0003-0292-2095 UNSPECIFIED Kudla, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-177206
DOI:	10.1002/1873-3468.13187
Journal or Publication Title:	FEBS Lett.
Volume:	592
Number:	15
Page Range:	S. 2582 - 2594
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1873-3468
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POSITIVE FEEDBACK-REGULATION; PROTEIN-KINASE; PLANT IMMUNITY; CELL-GROWTH; TIP GROWTH; ARABIDOPSIS; CALCIUM; CA2+; ACTIVATION; REVEALS Multiple languages Biochemistry & Molecular Biology; Biophysics; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17720