Universität zu Köln

Franck, Christina Maria ORCID: 0000-0003-3615-0871, Westermann, Jens ORCID: 0000-0001-9315-9691, Buerssner, Simon, Lentz, Roswitha, Lituiev, Dmytro Sergiiovych and Boisson-Dernier, Aurelien ORCID: 0000-0002-9790-3710 (2018). The Protein Phosphatases ATUNIS1 and ATUNIS2 Regulate Cell Wall Integrity in Tip-Growing Cells. Plant Cell, 30 (8). S. 1906 - 1924. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

Fast tip-growing plant cells such as pollen tubes (PTs) and root hairs (RHs) require a robust coordination between their internal growth machinery and modifications of their extracellular rigid, yet extensible, cell wall (CW). Part of this essential coordination is governed by members of the Catharanthus roseus receptor-like kinase1-like (CrRLK1L) subfamily of RLKs with FERONIA (FER) and its closest homologs, ANXUR1 (ANX1) and ANX2, controlling CW integrity during RH and PT growth, respectively. Recently, Leucine-Rich Repeat Extensin 8 (LRX8) to LRX11 were also shown to be important for CW integrity in PTs. We previously reported an anx1 anx2 suppressor screen in Arabidopsis thaliana that revealed MARIS (MRI) as a positive regulator of both FER- and ANX1/2-dependent CW integrity pathways. Here, we characterize a suppressor that exhibits a weak rescue of the anx1 anx2 PT bursting phenotype and a short RH phenotype. The corresponding suppressor mutation causes a D94N substitution in a Type One Protein Phosphatase we named ATUNIS1 (AUN1). We show that AUN1 and its closest homolog, AUN2, are nucleocytoplasmic negative regulators of tip growth. Moreover, we demonstrate that AUN1(D94N) and AUN1(H127A) harboring mutations in key amino acids of the conserved catalytic site of phosphoprotein phosphatases function as dominant amorphic variants that repress PT growth. Finally, genetic interaction studies using the hypermorph MRIR240C and amorph AUN1(D94N) dominant variants indicate that LRX8-11 and ANX1/2 function in distinct but converging pathways to fine-tune CW integrity during tip growth.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Franck, Christina Maria UNSPECIFIED orcid.org/0000-0003-3615-0871 UNSPECIFIED Westermann, Jens UNSPECIFIED orcid.org/0000-0001-9315-9691 UNSPECIFIED Buerssner, Simon UNSPECIFIED UNSPECIFIED UNSPECIFIED Lentz, Roswitha UNSPECIFIED UNSPECIFIED UNSPECIFIED Lituiev, Dmytro Sergiiovych UNSPECIFIED UNSPECIFIED UNSPECIFIED Boisson-Dernier, Aurelien UNSPECIFIED orcid.org/0000-0002-9790-3710 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-177531
DOI:	10.1105/tpc.18.00284
Journal or Publication Title:	Plant Cell
Volume:	30
Number:	8
Page Range:	S. 1906 - 1924
Date:	2018
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-298X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CATALYTIC SUBUNIT; FUNCTIONAL EXPRESSION; MUTATIONAL ANALYSIS; POLLEN GERMINATION; COMPLEX; IDENTIFICATION; GENERATION; GROWTH; 2A Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17753