Universität zu Köln

Erdogan, Alican J., Ali, Muna, Habich, Markus, Salscheider, Silja L., Schu, Laura, Petrungaro, Carmelina, Thomas, Luke W., Ashcroft, Margaret, Leichert, Lars I., Roma, Leticia Prates and Riemer, Jan (2018). The mitochondrial oxidoreductase CHCHD4 is present in a semi-oxidized state in vivo. Redox Biol., 17. S. 200 - 207. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 2213-2317

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Abstract

Disulfide formation in the mitochondrial intermembrane space is an essential process catalyzed by a disulfide relay machinery. In mammalian cells, the key enzyme in this machinery is the oxidoreductase CHCHD4/Mia40. Here, we determined the in vivo CHCHD4 redox state, which is the major determinant of its cellular activity. We found that under basal conditions, endogenous CHCHD4 redox state in cultured cells and mouse tissues was predominantly oxidized, however, degrees of oxidation in different tissues varied from 70% to 90% oxidized. To test whether differences in the ratio between CHCHD4 and ALR might explain tissue-specific differences in the CHCHD4 redox state, we determined the molar ratio of both proteins in different mouse tissues. Surprisingly, ALR is superstoichiometric over CHCHD4 in most tissues. However, the levels of CHCHD4 and the ratio of ALR over CHCHD4 appear to correlate only weakly with the redox state, and although ALR is present in super-stoichiometric amounts, it does not lead to fully oxidized CHCHD4.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Erdogan, Alican J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ali, Muna UNSPECIFIED UNSPECIFIED UNSPECIFIED Habich, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Salscheider, Silja L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schu, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Petrungaro, Carmelina UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Luke W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ashcroft, Margaret UNSPECIFIED UNSPECIFIED UNSPECIFIED Leichert, Lars I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roma, Leticia Prates UNSPECIFIED UNSPECIFIED UNSPECIFIED Riemer, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-180790
DOI:	10.1016/j.redox.2018.03.014
Journal or Publication Title:	Redox Biol.
Volume:	17
Page Range:	S. 200 - 207
Date:	2018
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	2213-2317
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DISULFIDE BOND FORMATION; INTERMEMBRANE SPACE; LIVER-REGENERATION; SULFHYDRYL OXIDASE; RESPIRATORY-CHAIN; ELECTRON-TRANSFER; RELAY SYSTEM; HUMAN MIA40; PROTEINS; IMPORT Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18079