Universität zu Köln

Kovacs, Levente ORCID: 0000-0002-3226-3740, Chao-Chu, Jennifer, Schneider, Sandra, Gottardo, Marco, Tzolovsky, George, Dzhindzhev, Nikola S., Riparbelli, Maria Giovanna, Callaini, Giuliano ORCID: 0000-0003-2252-0309 and Glover, David M. (2018). Gorab is a Golgi protein required for structure and duplication of Drosophila centrioles. Nature Genet., 50 (7). S. 1021 - 1034. NEW YORK: NATURE PUBLISHING GROUP. ISSN 1546-1718

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Abstract

We demonstrate that a Drosophila Golgi protein, Gorab, is present not only in the trans-Golgi but also in the centriole cartwheel where, complexed to Sas6, it is required for centriole duplication. In addition to centriole defects, flies lacking Gorab are uncoordinated due to defects in sensory cilia, which lose their nine-fold symmetry. We demonstrate the separation of centriole and Golgi functions of Drosophila Gorab in two ways: first, we have created Gorab variants that are unable to localize to trans-Golgi but can still rescue the centriole and cilia defects of gorab null flies; second, we show that expression of C-terminally tagged Gorab disrupts Golgi functions in cytokinesis of male meiosis, a dominant phenotype overcome by mutations preventing Golgi targeting. Our findings suggest that during animal evolution, a Golgi protein has arisen with a second, apparently independent, role in centriole duplication.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kovacs, Levente UNSPECIFIED orcid.org/0000-0002-3226-3740 UNSPECIFIED Chao-Chu, Jennifer UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Gottardo, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Tzolovsky, George UNSPECIFIED UNSPECIFIED UNSPECIFIED Dzhindzhev, Nikola S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Riparbelli, Maria Giovanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Callaini, Giuliano UNSPECIFIED orcid.org/0000-0003-2252-0309 UNSPECIFIED Glover, David M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-181759
DOI:	10.1038/s41588-018-0149-1
Journal or Publication Title:	Nature Genet.
Volume:	50
Number:	7
Page Range:	S. 1021 - 1034
Date:	2018
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	NEW YORK
ISSN:	1546-1718
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARTWHEEL-LESS CENTRIOLES; SINGLE PROCENTRIOLE; CENTROSOME; MICROTUBULES; CYTOKINESIS; NUCLEATION; ORGANIZES; MUTATIONS; COMPLEXES; ENCODES Multiple languages Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18175