Universität zu Köln

Koehler, Felix C., Cornely, Oliver A., Wisplinghoff, Hilmar, Schauss, Astrid C., Salmanton-Garcia, Jon ORCID: 0000-0002-6766-8297, Ostermann, Helmut, Ziegler, Maren, Bacher, Petra, Scheffold, Alexander ORCID: 0000-0002-0626-343X, Alex, Regina, Richter, Anne and Koehler, Philipp ORCID: 0000-0002-7386-7495 (2018). Candida-Reactive T Cells for the Diagnosis of Invasive Candida Infection-A Prospective Pilot Study. Front. Microbiol., 9. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-302X

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Abstract

Background: Blood or tissue culture or histology prove invasive Candida infection, but long time to result, limited feasibility and sensitivity call for new approaches. In this pilot project, we describe the diagnostic potential of quantitating Candida-reactive, CD4/CD69/CD154 positive lymphocytes in blood of patients with invasive Candida infection. Methods: We used flow cytometry quantitating Candida-reactive, CD4/CD69/CD154 positive lymphocytes from peripheral blood of patients with invasive Candida infection, from patients at risk and healthy volunteers as controls. Results: Elevated levels of Candida-reactive lymphocytes were measured in 13 patients with proven invasive Candida infection and in one patient with probable hepatosplenic candidiasis. Results of three candidemia patients were uninterpretable due to autofluorescence of samples. Twelve of 13 patients had Candida identified to species level by conventional methods, and T cell reactivity correctly identified Candida species in 10 of 12 patients. Nine hematological high-risk patients and 14 healthy donors had no elevated Candida-reactive T cell counts. Conclusions: This Candida-reactive lymphocyte assay correctly identified the majority of patients with invasive Candida infection and the respective species. Our assay has the potential to support diagnosis of invasive Candida infection to species level and to facilitate tailored treatment even when biopsies are contraindicated or cultures remain negative.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koehler, Felix C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cornely, Oliver A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wisplinghoff, Hilmar UNSPECIFIED UNSPECIFIED UNSPECIFIED Schauss, Astrid C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Salmanton-Garcia, Jon UNSPECIFIED orcid.org/0000-0002-6766-8297 UNSPECIFIED Ostermann, Helmut UNSPECIFIED UNSPECIFIED UNSPECIFIED Ziegler, Maren UNSPECIFIED UNSPECIFIED UNSPECIFIED Bacher, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheffold, Alexander UNSPECIFIED orcid.org/0000-0002-0626-343X UNSPECIFIED Alex, Regina UNSPECIFIED UNSPECIFIED UNSPECIFIED Richter, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Koehler, Philipp UNSPECIFIED orcid.org/0000-0002-7386-7495 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-182659
DOI:	10.3389/fmicb.2018.01381
Journal or Publication Title:	Front. Microbiol.
Volume:	9
Date:	2018
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-302X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FUNGAL-INFECTIONS; BLOOD CULTURES; DISEASES; MANAGEMENT; GUIDELINE; SOCIETY; ASSAY Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18265