Universität zu Köln

McMillan, Stephanie C., Zhang, Jing, Phan, Hue-Eileen, Jeradi, Shirine, Probst, Leona ORCID: 0000-0001-6226-1060, Hammerschmidt, Matthias and Akimenko, Marie-Andree (2018). A regulatory pathway involving retinoic acid and calcineurin demarcates and maintains joint cells and osteoblasts in regenerating fin. Development, 145 (11). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9129

Full text not available from this repository.

Abstract

During zebrafish fin regeneration, blastema cells lining the epidermis differentiate into osteoblasts and joint cells to reconstruct the segmented bony rays. We show that osteoblasts and joint cells originate from a common cell lineage, but are committed to different cell fates. Pre-osteoblasts expressing runx2a/b commit to the osteoblast lineage upon expressing sp7, whereas the strong upregulation of hoxa13a correlates with a commitment to a joint cell type. In the distal regenerate, hoxa13a, evx1 and pthlha are sequentially upregulated at regular intervals to define the newly identified presumptive joint cells. Presumptive joint cells mature into joint-forming cells, a distinct cell cluster that maintains the expression of these factors. Analysis of evx1 null mutants reveals that evx1 is acting upstream of pthlha and downstream of or in parallel with hoxa13a. Calcineurin activity, potentially through the inhibition of retinoic acid signaling, regulates evx1, pthlha and hoxa13a expression during joint formation. Furthermore, retinoic acid treatment induces osteoblast differentiation in mature joint cells, leading to ectopic bone deposition in joint regions. Overall, our data reveal a novel regulatory pathway essential for joint formation in the regenerating fin.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code McMillan, Stephanie C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Jing UNSPECIFIED UNSPECIFIED UNSPECIFIED Phan, Hue-Eileen UNSPECIFIED UNSPECIFIED UNSPECIFIED Jeradi, Shirine UNSPECIFIED UNSPECIFIED UNSPECIFIED Probst, Leona UNSPECIFIED orcid.org/0000-0001-6226-1060 UNSPECIFIED Hammerschmidt, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Akimenko, Marie-Andree UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-183266
DOI:	10.1242/dev.161158
Journal or Publication Title:	Development
Volume:	145
Number:	11
Date:	2018
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9129
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ZEBRAFISH CAUDAL FIN; INDIAN-HEDGEHOG; GENE-EXPRESSION; SONIC-HEDGEHOG; BLASTEMA FORMATION; ISOMETRIC GROWTH; CONNEXIN43 GJA1; PEPTIDE PTHRP; TGF-BETA; BONE Multiple languages Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18326