Universität zu Köln

Ahuja, Gaurav ORCID: 0000-0002-2837-9361, Reichel, Vera, Kowatschew, Daniel, Syed, Adnan S., Kotagiri, Aswani Kumar, Oka, Yuichiro, Weth, Franco and Korsching, Sigrun I. ORCID: 0000-0002-5450-172X (2018). Overlapping but distinct topology for zebrafish V2R-like olfactory receptors reminiscent of odorant receptor spatial expression zones. BMC Genomics, 19. LONDON: BIOMED CENTRAL LTD. ISSN 1471-2164

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Abstract

Background: The sense of smell is unrivaled in terms of molecular complexity of its input channels. Even zebrafish, a model vertebrate system in many research fields including olfaction, possesses several hundred different olfactory receptor genes, organized in four different gene families. For one of these families, the initially discovered odorant receptors proper, segregation of expression into distinct spatial subdomains within a common sensory surface has been observed both in teleost fish and in mammals. However, for the remaining three families, little to nothing was known about their spatial coding logic. Here we wished to investigate, whether the principle of spatial segregation observed for odorant receptors extends to another olfactory receptor family, the V2R-related OlfC genes. Furthermore we thought to examine, how expression of OlfC genes is integrated into expression zones of odorant receptor genes, which in fish share a single sensory surface with OlfC genes. Results: To select representative genes, we performed a comprehensive phylogenetic study of the zebrafish OlfC family, which identified a novel OlfC gene, reduced the number of pseudogenes to 1, and brought the total family size to 60 intact OlfC receptors. We analyzed the spatial pattern of OlfC-expressing cells for seven representative receptors in three dimensions (height within the epithelial layer, horizontal distance from the center of the olfactory organ, and height within the olfactory organ). We report non-random distributions of labeled neurons for all OlfC genes analysed. Distributions for sparsely expressed OlfC genes are significantly different from each other in nearly all cases, broad overlap notwithstanding. For two of the three coordinates analyzed, OlfC expression zones are intercalated with those of odorant receptor zones, whereas in the third dimension some segregation is observed. Conclusion: Our results show that V2R-related OlfC genes follow the same spatial logic of expression as odorant receptors and their expression zones intermingle with those of odorant receptor genes. Thus, distinctly different expression zones for individual receptor genes constitute a general feature shared by teleost and tetrapod V2R/OlfC and odorant receptor families alike.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ahuja, Gaurav UNSPECIFIED orcid.org/0000-0002-2837-9361 UNSPECIFIED Reichel, Vera UNSPECIFIED UNSPECIFIED UNSPECIFIED Kowatschew, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Syed, Adnan S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kotagiri, Aswani Kumar UNSPECIFIED UNSPECIFIED UNSPECIFIED Oka, Yuichiro UNSPECIFIED UNSPECIFIED UNSPECIFIED Weth, Franco UNSPECIFIED UNSPECIFIED UNSPECIFIED Korsching, Sigrun I. UNSPECIFIED orcid.org/0000-0002-5450-172X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-186019
DOI:	10.1186/s12864-018-4740-8
Journal or Publication Title:	BMC Genomics
Volume:	19
Date:	2018
Publisher:	BIOMED CENTRAL LTD
Place of Publication:	LONDON
ISSN:	1471-2164
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENE-EXPRESSION; EVOLUTIONARY DYNAMICS; EPITHELIUM; SYSTEM; MAMMALS; FAMILY; SEGREGATION; DOMAINS; MOUSE; MAIN Multiple languages Biotechnology & Applied Microbiology; Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18601