Universität zu Köln

Endepols, Heike ORCID: 0000-0002-6166-4818, Mottaghy, Felix M., Simsekyilmaz, Sakine, Bucerius, Jan, Vogt, Felix, Winz, Oliver, Richarz, Raphael, Krapf, Philipp ORCID: 0000-0003-1671-2443, Neumaier, Bernd ORCID: 0000-0001-5425-3116, Zlatopolskiy, Boris D. and Morgenroth, Agnieszka (2018). In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model. Sci Rep, 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

The short-and long-term success of intravascular stents depends on a proper re-endothelialisation after the intervention-induced endothelial denudation. The aim of this study was to evaluate the potential of in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with prostate-specific membrane antigen PSMA) expression as a marker of re-endothelialisation. Fifteen Sprague Dawley rats underwent unilateral balloon angioplasty of the common carotid artery (CCA). Positron emission tomography (PET) using the GCPII-targeting tracer [F-18] DCFPyL was performed after 5-21 days (scan 60120 min post injection). In two animals, the GCPII inhibitor PMPA (23 mg/kg BW) was added to the tracer solution. After PET, both CCAs were removed, dissected, and immunostained with the GCPII specific antibody YPSMA-1. Difference of GCPII expression between both CCAs was established by PCR analysis. [F-18] DCFPyL uptake was significantly higher in the ipsilateral compared to the contralateral CCA with an ipsi-/contralateral ratio of 1.67 +/- 0.39. PMPA blocked tracer binding. The selective expression of GCPII in endothelial cells of the treated CCA was confirmed by immunohistological staining. PCR analysis verified the site-specific GCPII expression. By using a molecular imaging marker of GCPII expression, we provide the first non-invasive in vivo delineation of re-endothelialisation after angioplasty.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Endepols, Heike UNSPECIFIED orcid.org/0000-0002-6166-4818 UNSPECIFIED Mottaghy, Felix M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Simsekyilmaz, Sakine UNSPECIFIED UNSPECIFIED UNSPECIFIED Bucerius, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Vogt, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Winz, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Richarz, Raphael UNSPECIFIED UNSPECIFIED UNSPECIFIED Krapf, Philipp UNSPECIFIED orcid.org/0000-0003-1671-2443 UNSPECIFIED Neumaier, Bernd UNSPECIFIED orcid.org/0000-0001-5425-3116 UNSPECIFIED Zlatopolskiy, Boris D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Morgenroth, Agnieszka UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-186531
DOI:	10.1038/s41598-018-25863-1
Journal or Publication Title:	Sci Rep
Volume:	8
Date:	2018
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MEMBRANE ANTIGEN-EXPRESSION; PROGENITOR CELLS; PET; MECHANISMS; DISEASE; STENT Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18653