Shai, Nadav ORCID: 0000-0002-2812-3884, Yifrach, Eden, van Roermund, Carlo W. T., Cohen, Nir, Bibi, Chen, Ijlst, Lodewijk, Cavellini, Laetitia ORCID: 0000-0003-1743-2689, Meurisse, Julie, Schuster, Ramona ORCID: 0000-0002-2227-3718, Zada, Lior, Mari, Muriel C., Reggiori, Fulvio M., Hughes, Adam L., Escobar-Henriques, Mafalda ORCID: 0000-0002-0879-3119, Cohen, Mickael M., Waterham, Hans R., Wanders, Ronald J. A., Schuldiner, Maya ORCID: 0000-0001-9947-115X and Zalckvar, Einat (2018). Systematic mapping of contact sites reveals tethers and a function for the peroxisome-mitochondria contact. Nat. Commun., 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

The understanding that organelles are not floating in the cytosol, but rather held in an organized yet dynamic interplay through membrane contact sites, is altering the way we grasp cell biological phenomena. However, we still have not identified the entire repertoire of contact sites, their tethering molecules and functions. To systematically characterize contact sites and their tethering molecules here we employ a proximity detection method based on split fluorophores and discover four potential new yeast contact sites. We then focus on a little-studied yet highly disease-relevant contact, the Peroxisome-Mitochondria (PerMit) proximity, and uncover and characterize two tether proteins: Fzo1 and Pex34. We genetically expand the PerMit contact site and demonstrate a physiological function in beta-oxidation of fatty acids. Our work showcases how systematic analysis of contact site machinery and functions can deepen our understanding of these structures in health and disease.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Shai, NadavUNSPECIFIEDorcid.org/0000-0002-2812-3884UNSPECIFIED
Yifrach, EdenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van Roermund, Carlo W. T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cohen, NirUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bibi, ChenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ijlst, LodewijkUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cavellini, LaetitiaUNSPECIFIEDorcid.org/0000-0003-1743-2689UNSPECIFIED
Meurisse, JulieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuster, RamonaUNSPECIFIEDorcid.org/0000-0002-2227-3718UNSPECIFIED
Zada, LiorUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mari, Muriel C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Reggiori, Fulvio M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hughes, Adam L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Escobar-Henriques, MafaldaUNSPECIFIEDorcid.org/0000-0002-0879-3119UNSPECIFIED
Cohen, Mickael M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Waterham, Hans R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wanders, Ronald J. A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuldiner, MayaUNSPECIFIEDorcid.org/0000-0001-9947-115XUNSPECIFIED
Zalckvar, EinatUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-186712
DOI: 10.1038/s41467-018-03957-8
Journal or Publication Title: Nat. Commun.
Volume: 9
Date: 2018
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BIMOLECULAR FLUORESCENCE COMPLEMENTATION; COMBINED TRANSMEMBRANE TOPOLOGY; PROTEIN-PROTEIN INTERACTIONS; SIGNAL PEPTIDE PREDICTION; SACCHAROMYCES-CEREVISIAE; PLASMA-MEMBRANE; YEAST LIBRARIES; ER; FUSION; MITOFUSINMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18671

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