Guo, Tiannan ORCID: 0000-0003-3869-7651, Li, Li, Zhong, Qing ORCID: 0000-0002-5340-301X, Rupp, Niels J., Charmpi, Konstantina, Wong, Christine E., Wagner, Ulrich, Rueschoff, Jan H., Jochum, Wolfram, Fankhauser, Christian Daniel, Saba, Karim, Poyet, Cedric, Wild, Peter J., Aebersold, Ruedi and Beyer, Andreas ORCID: 0000-0002-3891-2123 (2018). Multi-region proteome analysis quantifies spatial heterogeneity of prostate tissue biomarkers. Life Sci. Alliance, 1 (2). COLD SPRING HARBOR: LIFE SCIENCE ALLIANCE LLC. ISSN 2575-1077

Full text not available from this repository.

Abstract

It remains unclear to what extent tumor heterogeneity impacts on protein biomarker discovery. Here, we quantified proteome intra-tissue heterogeneity (ITH) based on a multi-region analysis of prostate tissues using pressure cycling technology and Sequential Windowed Acquisition of all THeoretical fragment ion mass spectrometry. We quantified 6,873 proteins and analyzed the ITH of 3,700 proteins. The level of ITH varied depending on proteins and tissue types. Benign tissues exhibited more complex ITH patterns than malignant tissues. Spatial variability of 10 prostate biomarkers was validated by immunohistochemistry in an independent cohort (n = 83) using tissue microarrays. Prostate-specific antigen was preferentially variable in benign prostatic hyperplasia, whereas growth/differentiation factor 15 substantially varied in prostate adenocarcinomas. Furthermore, we found that DNA repair pathways exhibited a high degree of variability in tumorous tissues, which may contribute to the genetic heterogeneity of tumors. This study conceptually adds a new perspective to protein biomarker discovery: it suggests that recent technological progress should be exploited to quantify and account for spatial proteome variation to complement biomarker identification and utilization.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Guo, TiannanUNSPECIFIEDorcid.org/0000-0003-3869-7651UNSPECIFIED
Li, LiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhong, QingUNSPECIFIEDorcid.org/0000-0002-5340-301XUNSPECIFIED
Rupp, Niels J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Charmpi, KonstantinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wong, Christine E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wagner, UlrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rueschoff, Jan H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jochum, WolframUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fankhauser, Christian DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saba, KarimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Poyet, CedricUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wild, Peter J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Aebersold, RuediUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beyer, AndreasUNSPECIFIEDorcid.org/0000-0002-3891-2123UNSPECIFIED
URN: urn:nbn:de:hbz:38-186721
DOI: 10.26508/lsa.201800042
Journal or Publication Title: Life Sci. Alliance
Volume: 1
Number: 2
Date: 2018
Publisher: LIFE SCIENCE ALLIANCE LLC
Place of Publication: COLD SPRING HARBOR
ISSN: 2575-1077
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
INTRATUMOR HETEROGENEITY; REACTIVE STROMA; CANCER; CARCINOMA; EVOLUTION; CELLS; SIGNATURES; DISCOVERY; DIAGNOSIS; PROGNOSISMultiple languages
BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18672

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item