Universität zu Köln

Rippin, Martin ORCID: 0000-0003-4362-0122, Borchhardt, Nadine, Williams, Laura, Colesie, Claudia ORCID: 0000-0003-1136-0290, Jung, Patrick ORCID: 0000-0002-7607-3906, Buedel, Burkhard, Karsten, Ulf and Becker, Burkhard ORCID: 0000-0002-7965-1396 (2018). Genus richness of microalgae and Cyanobacteria in biological soil crusts from Svalbard and Livingston Island: morphological versus molecular approaches. Polar Biol., 41 (5). S. 909 - 924. NEW YORK: SPRINGER. ISSN 1432-2056

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Abstract

Biological soil crusts (BSCs) are key components of polar ecosystems. These complex communities are important for terrestrial polar habitats as they include major primary producers that fix nitrogen, prevent soil erosion and can be regarded as indicators for climate change. To study the genus richness of microalgae and Cyanobacteria in BSCs, two different methodologies were employed and the outcomes were compared: morphological identification using light microscopy and the annotation of ribosomal sequences taken from metatranscriptomes. The analyzed samples were collected from Ny-Alesund, Svalbard, Norway, and the Juan Carlos I Antarctic Base, Livingston Island, Antarctica. This study focused on the following taxonomic groups: Klebsormidiophyceae, Chlorophyceae, Trebouxiophyceae, Xanthophyceae and Cyanobacteria. In total, combining both approaches, 143 and 103 genera were identified in the Arctic and Antarctic samples, respectively. Furthermore, both techniques concordantly determined 15 taxa in the Arctic and 7 taxa in the Antarctic BSC. In general, the molecular analysis indicated a higher microalgal and cyanobacterial genus richness (about 11 times higher) than the morphological approach. In terms of eukaryotic algae, the two sampling sites displayed comparable genus counts while the cyanobacterial genus richness was much higher in the BSC from Ny-Alesund. For the first time, the presence of the genera Chloroidium, Ankistrodesmus and Dunaliella in polar regions was determined by the metatranscriptomic analysis. Overall, these findings illustrate that only the combination of morphological and molecular techniques, in contrast to one single approach, reveals higher genus richness for complex communities such as polar BSCs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rippin, Martin UNSPECIFIED orcid.org/0000-0003-4362-0122 UNSPECIFIED Borchhardt, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Williams, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Colesie, Claudia UNSPECIFIED orcid.org/0000-0003-1136-0290 UNSPECIFIED Jung, Patrick UNSPECIFIED orcid.org/0000-0002-7607-3906 UNSPECIFIED Buedel, Burkhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Karsten, Ulf UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Burkhard UNSPECIFIED orcid.org/0000-0002-7965-1396 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-187162
DOI:	10.1007/s00300-018-2252-2
Journal or Publication Title:	Polar Biol.
Volume:	41
Number:	5
Page Range:	S. 909 - 924
Date:	2018
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-2056
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SOUTH-SHETLAND ISLANDS; COCCOID GREEN-ALGAE; KING-GEORGE-ISLAND; PHYLOGENETIC-RELATIONSHIPS; TAXONOMIC REVISION; GROWTH; CHLOROPHYTA; DIVERSITY; TREBOUXIOPHYCEAE; BIODIVERSITY Multiple languages Biodiversity Conservation; Ecology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18716