Universität zu Köln

Schulz, Alexandra, Fuchs, Paul Christian, Hans, Nadine, Oplaender, Christian, Valdez, Leandra Boerner and Schiefer, Jennifer Lynn ORCID: 0000-0002-3959-9394 (2018). Inhibition of Bromelain Activity During Enzymatic Debridement of Burn Wounds Pretreated With Frequently Used Products. J. Burn Care Res., 39 (3). S. 413 - 423. OXFORD: OXFORD UNIV PRESS. ISSN 1559-0488

Full text not available from this repository.

Abstract

An enzyme mixture containing bromelain (NexoBrid (R)) was found to be suitable for enzymatic debridement of burn wounds, as determined by the criteria of patient comfort and pain, selectivity, and efficiency. Nevertheless, daily experience showed that pretreatment of burn wounds with several other clinical agents may inhibit debridement efficiency. Therefore, the current study was performed to identify those agents and evaluate their debridement inhibition capabilities. The impact of several common agents as well pH, on NexoBrid (R) debridement efficiency was evaluated in vitro. A collagen-based dermal substitute (MatriDerm (R)) was exposed to NexoBrid (R) in the presence of different agents of varying concentrations. Digestion was documented. The criteria used for judging digestion were independently classified by 3 investigators at least 3 times in succession. When a low concentration (1.0 mg/ml) of NexoBrid (R) was used, a >= 50% concentration of Prontosan (R) had an impact on enzymatic activity. Comparable results were obtained when even lower concentrations of Octenisept (R) (>= 10%) were used. A 100-mu mol/L concentration of copper inhibited the enzymatic activity of both a low (1.0 mg/ml) and high (10 mg/ml) concentration of NexoBrid (R). Silver-sulfadiazine at concentrations of 10% and 90% inhibited the activity of 1 mg/ml NexoBrid (R). No complete inhibition of NexoBrid (R) activity occurred at any concentration of iron. We recommend using polyhexanide-containing agents (Prontosan (R)) to rinse and presoak burn wounds. Pretreatment of burn wounds with agents containing silver and copper should be avoided. Experimentally, we found a partial inhibition of NexoBrid (R) activity at the distinct pH values of 3 and 11.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schulz, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuchs, Paul Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Hans, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Oplaender, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Valdez, Leandra Boerner UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiefer, Jennifer Lynn UNSPECIFIED orcid.org/0000-0002-3959-9394 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-188474
DOI:	10.1097/BCR.0000000000000609
Journal or Publication Title:	J. Burn Care Res.
Volume:	39
Number:	3
Page Range:	S. 413 - 423
Date:	2018
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1559-0488
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CERIUM NITRATE; INJURY; AGENT; MANAGEMENT; EFFICACY; OCTENISEPT(R); POLIHEXANIDE; INFLAMMATION; STABILITY; THERAPY Multiple languages Critical Care Medicine; Dermatology; Surgery Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18847