Universität zu Köln

Rangarajan, Aathmaja Anandhi and Schnetz, Karin ORCID: 0000-0003-4693-6202 (2018). Interference of transcription across H-NS binding sites and repression by H-NS. Mol. Microbiol., 108 (3). S. 226 - 240. HOBOKEN: WILEY. ISSN 1365-2958

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Abstract

Nucleoid-associated protein H-NS represses transcription by forming extended DNA-H-NS complexes. Repression by H-NS operates mostly at the level of transcription initiation. Less is known about how DNA-H-NS complexes interfere with transcription elongation. In vitro H-NS has been shown to enhance RNA polymerase pausing and to promote Rho-dependent termination, while in vivo inhibition of Rho resulted in a decrease of the genome occupancy by H-NS. Here we show that transcription directed across H-NS binding regions relieves H-NS (and H-NS/StpA) mediated repression of promoters in these regions. Further, we observed a correlation of transcription across the H-NS-bound region and de-repression. The data suggest that the transcribing RNA polymerase is able to remodel the H-NS complex and/or dislodge H-NS from the DNA and thus relieve repression. Such an interference of transcription and H-NS mediated repression may imply that poorly transcribed AT-rich loci are prone to be repressed by H-NS, while efficiently transcribed loci escape repression.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rangarajan, Aathmaja Anandhi UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnetz, Karin UNSPECIFIED orcid.org/0000-0003-4693-6202 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-188569
DOI:	10.1111/mmi.13926
Journal or Publication Title:	Mol. Microbiol.
Volume:	108
Number:	3
Page Range:	S. 226 - 240
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2958
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ESCHERICHIA-COLI K-12; NUCLEOID-ASSOCIATED PROTEINS; RNA-POLYMERASE; BGL OPERON; STRUCTURAL BASIS; GENE-EXPRESSION; TERMINATION EFFICIENCY; MEDIATED REPRESSION; REGULATORY ELEMENT; FOREIGN DNA Multiple languages Biochemistry & Molecular Biology; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18856