Universität zu Köln

Karsten, Maria Margarete ORCID: 0000-0001-7587-7550, Enders, Sabine, Knabl, Julia, Kirn, Verena, Duewell, Peter ORCID: 0000-0003-0836-6448, Rack, Brigitte, Blohmer, Jens-Uwe, Mayr, Doris and Dian, Darius (2018). Biologic meshes and synthetic meshes in cancer patients: a double-edged sword: differences in production of IL-6 and IL-12 caused by acellular dermal matrices in human immune cells. Arch. Gynecol. Obstet., 297 (5). S. 1265 - 1271. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1432-0711

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Abstract

In 2005, Breuing et al. first described the use of acellular dermal matrices (ADMs) in breast cancer patients. ADMs are assumed to be safe to use in an oncologic setting, but data from controlled studies are still needed. Here, we investigate the effects of ADMs on the production of interleukin (IL)-6 and IL-12, key regulators of immune suppression and activation. Strattice (ST), CollaMend (CM), and Biodesign (BD) biologic meshes and TiLoop, a synthetic mesh (TL), were used in this study. We isolated myeloid dendritic cells (MDCs), untouched plasmacytoid dendritic cells (pDCs), na < ve B cells, and CD8+ T cells and co-cultured these cells with either the biologic meshes or TL. As positive controls, we used CpG ODN 2216 or lipopolysaccharide (LPS). The cytokine concentrations of IL-12p70 and IL-6 were determined after 7 days using sandwich ELISA sets. There were highly significant differences between the ADMs and TL in terms of their ability to stimulate immunologic responses. IL-6 expression was significantly increased in B cells (p = 0.0006131) and T cells (p = 0.00418) when comparing TL and ADMs. We also identified significant differences in IL-12 production by B cells (p = 0.0166) and T cells (p = 0.003636) when comparing TL and ADMs. Despite the assumed lack of an immunological response to ADMs, in our experimental study, human immune cells reacted with significantly different cytokine profiles. These findings may have implications for the potential activation or suppression of effector cells in cancer patients and could explain some of the post clinical post surgical signs of ADMS like skin rush and seroma.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Karsten, Maria Margarete UNSPECIFIED orcid.org/0000-0001-7587-7550 UNSPECIFIED Enders, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Knabl, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Kirn, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Duewell, Peter UNSPECIFIED orcid.org/0000-0003-0836-6448 UNSPECIFIED Rack, Brigitte UNSPECIFIED UNSPECIFIED UNSPECIFIED Blohmer, Jens-Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Mayr, Doris UNSPECIFIED UNSPECIFIED UNSPECIFIED Dian, Darius UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-188639
DOI:	10.1007/s00404-018-4710-z
Journal or Publication Title:	Arch. Gynecol. Obstet.
Volume:	297
Number:	5
Page Range:	S. 1265 - 1271
Date:	2018
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
ISSN:	1432-0711
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MEMORY T-CELLS; BREAST RECONSTRUCTION; CLINICAL-TRIAL; IFN-GAMMA; IN-VITRO; INTERLEUKIN-12; ACTIVATION; CYTOKINE; PURIFICATION; LYMPHOCYTES Multiple languages Obstetrics & Gynecology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18863