Dodzian, Joanna, Kean, Sam, Seidel, Jens and Valenzano, Dario Riccardo (2018). A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri). J. Vis. Exp. (134). CAMBRIDGE: JOURNAL OF VISUALIZED EXPERIMENTS. ISSN 1940-087X

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Abstract

The development of husbandry practices in non-model laboratory fish used for experimental purposes has greatly benefited from the establishment of reference fish model systems, such as zebrafish and medaka. In recent years, an emerging fish - the turquoise killifish (Nothobranchius furzeri) - has been adopted by a growing number of research groups in the fields of biology of aging and ecology. With a captive life span of 4 - 8 months, this species is the shortest-lived vertebrate raised in captivity and allows the scientific community to test - in a short time - experimental interventions that can lead to alterations of the aging rate and life expectancy. Given the unique biology of this species, characterized by embryonic diapause, explosive sexual maturation, marked morphological and behavioral sexual dimorphism - and their relatively short adult life span - ad hoc husbandry practices are in urgent demand. This protocol reports a set of key husbandry measures that allow optimal turquoise killifish laboratory care, enabling the scientific community to adopt this species as a powerful laboratory animal model.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Dodzian, JoannaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kean, SamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Seidel, JensUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Valenzano, Dario RiccardoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-190262
DOI: 10.3791/57073
Journal or Publication Title: J. Vis. Exp.
Number: 134
Date: 2018
Publisher: JOURNAL OF VISUALIZED EXPERIMENTS
Place of Publication: CAMBRIDGE
ISSN: 1940-087X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SHORT-LIVED FISH; LIFE-SPAN; VERTEBRATE MODEL; GENOME; EVOLUTION; INSIGHTSMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/19026

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